Home / Journals / BIOCELL / Vol.37, No.2, 2013
Table of Content
  • Open Access

    ARTICLE

    Age related interaction of dopamine and serotonin synthesis in striatal synaptosomes

    MANOOCHEHR MESSRIPOUR1, AZADEH MESRIPOUR2
    BIOCELL, Vol.37, No.2, pp. 17-21, 2013, DOI:10.32604/biocell.2013.37.017
    Abstract Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during 25 min was measured by HPLC, employing electrochemical detection. Dopamine synthesis in synaptosomes… More >

  • Open Access

    ARTICLE

    Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test

    VIVIANE DAL-SOUTO FRESCURA1, ANDRIELLE WOUTERS KUHN1, HAYWOOD DAIL LAUGHINGHOUSE IV2, JUÇARA TEREZINHA PARANHOS1, SOLANGE BOSIO TEDESCO1
    BIOCELL, Vol.37, No.2, pp. 23-28, 2013, DOI:10.32604/biocell.2013.37.023
    Abstract Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared… More >

  • Open Access

    ARTICLE

    Confocal laser scanning microscopy and immunohistochemistry of cerebellar Lugaro cells

    Orlando J. CASTEJÓN
    BIOCELL, Vol.37, No.2, pp. 29-36, 2013, DOI:10.32604/biocell.2013.37.029
    Abstract The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fi bers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors… More >

  • Open Access

    ARTICLE

    Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry

    Solange Marta Franzói De MORAES, Thais Andréia BROGIO, Jacqueline Nelisis ZANONi, Mariana Cristina Vicente Umada ZAPATER, Sidney Barnabé PEREs, Luzmarina HERNANDES
    BIOCELL, Vol.37, No.2, pp. 37-43, 2013, DOI:10.32604/biocell.2013.37.037
    Abstract Creatine is widely used by athletes as an ergogenic resource. The aim of this study was to evaluate the influence of creatine supplementation on the duodenum of rats submitted to physical training. The number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were evaluated. Control animals received a standard chow for 8 weeks, and the treated ones received the standard chow for 4 weeks and were later fed with the same chow but added with 2% creatine. Animals were divided in groups: sedentary, sedentary supplemented with creatine, trained and trained supplemented with creatine. The training consisted… More >

  • Open Access

    ARTICLE

    Autophagy, apoptosis and organelle features during cell exposure to cadmiumč

    Cristiane Dos Santos VERGILIO, Edésio José Tenório De MELO*
    BIOCELL, Vol.37, No.2, pp. 45-54, 2013, DOI:10.32604/biocell.2013.37.045
    Abstract Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 μM – 20 μM) and exposure times (2 h – 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 μM… More >

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