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Marcelo de Castro Pazos*, Cristina Pacheco-Soares*, Newton Soares da Silva*, R.A. DaMatta**, M.T.T. Pacheco*
BIOCELL, Vol.27, No.3, pp. 301-309, 2003, DOI:10.32604/biocell.2003.27.301
Abstract The effects of Photodynamic Therapy using 2^nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2^nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/ cm² (Power=26mW; λ=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS₄) photodynamic treatment. The Aluminum… More >

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LUIS A. CARO¹, NATALIA SANTECCHIA¹, PABLO A. MARINANGELI¹, NÉSTOR R. CURVETTO^1,2, LUIS F. HERNÁNDEZ^1,3
BIOCELL, Vol.27, No.3, pp. 311-318, 2003, DOI:10.32604/biocell.2003.27.311
Abstract The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L^-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with… More >

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CARMINDA DA CRUZ-LANDIM*, FÁBIO CAMARGO ABDALLA*, MARIA ALICE DA CRUZ-HÖFLING**
BIOCELL, Vol.27, No.3, pp. 319-328, 2003, DOI:10.32604/biocell.2003.27.319
Abstract The spermatogenesis of Piaractus mesopotamicus was investigated under light and transmission electron microscopy. The specimens were captured from their natural environment (Rio Miranda and Rio Aquidauana, Pantanal Matogrossense, Brazil) during April and September. The results were compared with the spermatogenic data of specimens under captivity condition. In both conditions, P. mesopotamicus presented the typical spermatogenesis pattern of the teleost fishes, showing no significative differences. The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type… More >

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ALBERTO J. SOLARI*, MONICA I. RAHN*, ALICIA SAURA**, HUGO D. LUJAN**
BIOCELL, Vol.27, No.3, pp. 329-346, 2003, DOI:10.32604/biocell.2003.27.329
Abstract The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs… More >

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LUZMARINA HERNANDES*, LUCIENI CRISTINA MARQUES DA SILVA PEREIRA*, ELIANA PARISI ALVARES**
BIOCELL, Vol.27, No.3, pp. 347-351, 2003, DOI:10.32604/biocell.2003.27.347
Abstract The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and those where BAC was applied… More >

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ADELINA FERREIRA, HEIDI DOLDER
BIOCELL, Vol.27, No.3, pp. 353-362, 2003, DOI:10.32604/biocell.2003.27.353
Abstract Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the proacrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation.… More >

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OFELIA ACOSTA DE PÉREZ¹, LAURA LEIVA DE VILA², MARÍA ELISA PEICHOTO², SILVANA MARUÑAK³, RAQUEL RUÍZ³, PAMELA TEIBLER³, CAROLINA GAY², LAURA REY⁴
BIOCELL, Vol.27, No.3, pp. 363-370, 2003, DOI:10.32604/biocell.2003.27.363
Abstract Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 µl of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 µl of PBS. Two hours after injection mice were killed… More >

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