Home / Journals / BIOCELL / Vol.27, No.3, 2003
Table of Content
  • Open Access

    ARTICLE

    Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation

    Marcelo de Castro Pazos*, Cristina Pacheco-Soares*, Newton Soares da Silva*, R.A. DaMatta**, M.T.T. Pacheco*
    BIOCELL, Vol.27, No.3, pp. 301-309, 2003, DOI:10.32604/biocell.2003.27.301
    Abstract The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/ cm2 (Power=26mW; λ=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4) photodynamic treatment. The Aluminum… More >

  • Open Access

    ARTICLE

    Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina

    LUIS A. CARO1, NATALIA SANTECCHIA1, PABLO A. MARINANGELI1, NÉSTOR R. CURVETTO1,2, LUIS F. HERNÁNDEZ1,3
    BIOCELL, Vol.27, No.3, pp. 311-318, 2003, DOI:10.32604/biocell.2003.27.311
    Abstract The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with… More >

  • Open Access

    ARTICLE

    Morphological study of the spermatogenesis in the teleost Piaractus mesopotamicus

    CARMINDA DA CRUZ-LANDIM*, FÁBIO CAMARGO ABDALLA*, MARIA ALICE DA CRUZ-HÖFLING**
    BIOCELL, Vol.27, No.3, pp. 319-328, 2003, DOI:10.32604/biocell.2003.27.319
    Abstract The spermatogenesis of Piaractus mesopotamicus was investigated under light and transmission electron microscopy. The specimens were captured from their natural environment (Rio Miranda and Rio Aquidauana, Pantanal Matogrossense, Brazil) during April and September. The results were compared with the spermatogenic data of specimens under captivity condition. In both conditions, P. mesopotamicus presented the typical spermatogenesis pattern of the teleost fishes, showing no significative differences. The spermatozoon was classified as type I, which has a globular head without acrosome, a short middle piece and a long tail constituted only by the flagellum. This type of spermatozoon is considered the basic type… More >

  • Open Access

    ARTICLE

    A unique mechanism of nuclear division in Giardia lamblia involves components of the ventral disk and the nuclear envelope

    ALBERTO J. SOLARI*, MONICA I. RAHN*, ALICIA SAURA**, HUGO D. LUJAN**
    BIOCELL, Vol.27, No.3, pp. 329-346, 2003, DOI:10.32604/biocell.2003.27.329
    Abstract The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs… More >

  • Open Access

    ARTICLE

    Goblet cell number in the ileum of rats denervated during suckling and weaning

    LUZMARINA HERNANDES*, LUCIENI CRISTINA MARQUES DA SILVA PEREIRA*, ELIANA PARISI ALVARES**
    BIOCELL, Vol.27, No.3, pp. 347-351, 2003, DOI:10.32604/biocell.2003.27.347
    Abstract The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and those where BAC was applied… More >

  • Open Access

    ARTICLE

    Sperm ultrastructure and spermatogenesis in the lizard, Tropidurus itambere

    ADELINA FERREIRA, HEIDI DOLDER
    BIOCELL, Vol.27, No.3, pp. 353-362, 2003, DOI:10.32604/biocell.2003.27.353
    Abstract Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the proacrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation.… More >

  • Open Access

    ARTICLE

    Edematogenic and myotoxic activities of the duvernoy’s gland secretion of Philodryas olfersii from the north-east region of Argentina

    OFELIA ACOSTA DE PÉREZ1, LAURA LEIVA DE VILA2, MARÍA ELISA PEICHOTO2, SILVANA MARUÑAK3, RAQUEL RUÍZ3, PAMELA TEIBLER3, CAROLINA GAY2, LAURA REY4
    BIOCELL, Vol.27, No.3, pp. 363-370, 2003, DOI:10.32604/biocell.2003.27.363
    Abstract Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 µl of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 µl of PBS. Two hours after injection mice were killed… More >

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