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Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation

Marcelo de Castro Pazos*, Cristina Pacheco-Soares*, Newton Soares da Silva*, R.A. DaMatta**, M.T.T. Pacheco*
* Laboratório de Biologia Celular e Tecidual, Instituto de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, CEP 12244-000, São José dos Campos, São Paulo, Brazil.
** Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, CEP 28015-620, Campos dos Goytacazes, Rio de Janeiro, Brazil.
Address correspondence to: Prof. Dr. Newton Soares da Silva. Laboratório de Pesquisa e Desenvolvimento, Universidade do Vale do Paraíba, Av. Shishima Hifumi, 2911, CEP 12244-000, Sao José dos Campos, Sao Paulo, BRAZIL. Phone: (55-12) 3947-1143; Fax: (55-12) 3947-1149; E-mail: nsoares@univap.br

BIOCELL 2003, 27(3), 301-309. https://doi.org/10.32604/biocell.2003.27.301

Abstract

The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/ cm2 (Power=26mW; λ=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4) photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.

Keywords

Photodynamic therapy, phthalocyanines, laser, cell death, ultrastructure.

Cite This Article

De, M. (2003). Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation. BIOCELL, 27(3), 301–309.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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