Home / Journals / BIOCELL / Vol.28, No.3, 2004
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  • Open Access

    ARTICLE

    Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development

    GRACIELA CRISTINA CALABRESE*, ROSA WAINSTOK**
    BIOCELL, Vol.28, No.3, pp. 251-258, 2004, DOI:10.32604/biocell.2004.28.251
    Abstract Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages.
    Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein… More >

  • Open Access

    ARTICLE

    Differentiation and morphogenesis of Triatoma infestans (Klug 1834) female gonads. I – Post embryonic development

    CECILIA I. IBAÑEZ DE BARRETT, JUAN PABLO BOZZINI, MARTA MARIANO DE BOZZINI*
    BIOCELL, Vol.28, No.3, pp. 259-269, 2004, DOI:10.32604/biocell.2004.28.259
    Abstract The post-embryonic development of the female gonads in Triatoma infestans (Hemiptera, Heteroptera), insects of importance in health affairs as harbors and vectors of different tripanosomatidea flagellates, is presented in a complete follow-up since insect hatches from the egg up to the last molt in the fifth instar stage. The detailed description of the morphological changes which occur in each instar as well as careful measurements evaluating its size increase have been analyzed by stereomicroscopy, phase contrast, dark field, and oblique illumination, in order to optimize the observations as well the photographic register of gonad morphology and structure.
    The analysis… More >

  • Open Access

    ARTICLE

    Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes

    PABLO HORACIO MASEDA1, JORGE HUGO LEMCOFF1, MERCEDES MURÚA2, NORA FRAYSSINET3, MARTA SUSANA CARCELLER1†
    BIOCELL, Vol.28, No.3, pp. 271-277, 2004, DOI:10.32604/biocell.2004.28.271
    Abstract Lycium chilense, a deciduous perennial shrub, is one of the endangered native species of Patagonia due to sheep overgrazing. Chances of recolonization by seeds are scarce due to the limited density of propagules in the soil and very specific requirements for germination. The objective was to develop an in vitro propagation protocol that would help to perform reestablishment of this species in degraded areas of the Patagonian steppe. Seeds came from two provenances with different somatic number due to differences in ploidy level. Defoliated microcuttings were planted in test tubes with different growing media and taken to a growth chamber.… More >

  • Open Access

    ARTICLE

    Karyotype description of Pomacea patula catemacensis (Caenogastropoda, Ampullariidae), with an assessment of the taxonomic status of Pomacea patula

    MARÍA ESTHER DIUPOTEX-CHONG1, NÉSTOR J. CAZZANIGA2, ALEJANDRA HERNÁNDEZ-SANTOYO3, JOSÉ MIGUEL BETANCOURT-RULE4
    BIOCELL, Vol.28, No.3, pp. 279-285, 2004, DOI:10.32604/biocell.2004.28.279
    Abstract Mitotic chromosomes of the freshwater snail Pomacea patula catemacensis (Baker 1922) were analyzed on gill tissue of specimens from the type locality (Lake Catemaco, Mexico). The diploid number of chromosomes is 2n = 26, including nine metacentric and four submetacentric pairs; therefore, the fundamental number is FN = 52. No sex chromosomes could be identified. The same chromosome number and morphology were already reported for P. flagellata, i.e., the other species of the genus living in Mexico. The basic haploid number for family Ampullariidae was reported to be n° =°14 in the literature; so, its reduction to n° =°13 is… More >

  • Open Access

    ARTICLE

    RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct

    PABLO A. VALDECANTOS, MARTÍN E. ARGAÑARAZ, CARLOS M. ABATE, DORA C. MICELI
    BIOCELL, Vol.28, No.3, pp. 287-297, 2004, DOI:10.32604/biocell.2004.28.287
    Abstract As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this… More >

  • Open Access

    ARTICLE

    Protein detection in spermatids and spermatozoa of the butterfly Euptoieta hegesia (Lepidoptera)

    KARINA MANCINI, HEIDI DOLDER
    BIOCELL, Vol.28, No.3, pp. 299-310, 2004, DOI:10.32604/biocell.2004.28.299
    Abstract This study was undertaken to detect protein components in both sperm types of the butterfly Euptoieta hegesia. These spermatozoa possess complex extracellular structures for which the composition and functional significance are still unclear. In the apyrene sperm head, the proteic cap presented an external ring and an internal dense content; basic proteins were detected only in external portions. In the tail, the paracrystalline core of mitochondrial derivatives and the axoneme are rich in proteins. The extratesticular spermatozoa are covered by a proteic coat, which presented two distinct layers. In eupyrene spermatozoa, acrosome and nucleus were negatively stained, probably because of… More >

  • Open Access

    ARTICLE

    Brief Note : Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen

    M. I. ROSATTI, M. T. BECONI, M. CÓRDOBA
    BIOCELL, Vol.28, No.3, pp. 311-316, 2004, DOI:10.32604/biocell.2004.28.311
    Abstract Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed… More >

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