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Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers

ZONG-YUN LI1,2*, MEI-LI FU1, FANG-FANG HU1, SHU-FENG HUANG1, YUN-CHUN SONG2
1. School of Life Science, Xuzhou Normal University, Xuzhou 221116, China;
2. Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China.
Address correspondence to: Zong-Yun Li. School of Life Science, Xuzhou Normal University, Xuzhou 221116, CHINA. E-mail: zongyunli@yahoo.com.cn or zongyunli@hotmail.com

BIOCELL 2006, 30(1), 27-32. https://doi.org/10.32604/biocell.2006.30.027

Abstract

The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also vary through the cell cycle in rice. On extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers.

Keywords

cell cycle, fluorescent in situ hybridization (FISH), ribosomal DNA (45S rDNA), pachytene chromosomes, indica rice

Cite This Article

LI, Z., FU, M., HU, F., HUANG, S., SONG, Y. (2006). Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers. BIOCELL, 30(1), 27–32.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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