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Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods

VIVIANE DAL-SOUTO FRESCURA1, HAYWOOD DAIL LAUGHINGHOUSE IV2, THAIS SCOTTI DO CANTODOROW1, SOLANGE BOSIO TEDESCO1*
1. Laboratory of Cytogenetics and Genotoxicity, Department of Biology, Centro de Ciências Naturais e Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Avenida Roraima, nº 1000, Cep. 97105-900, Camobi, Santa Maria, Rio Grande do Sul, Brasil.
2. Centre for Protein Engineering, Institute of Chemistry B6, University of Liège, Belgium.
*Address correspondence to: Solange Bosio Tedesco. E-mail:

BIOCELL 2012, 36(3), 143-145. https://doi.org/10.32604/biocell.2012.36.143

Abstract

Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of P. paniculata using the Alexander’s stain, which proved the most adequate method to estimate pollen viability.

Keywords

pollen grains, barba-de-bode, acetic orcein, acetic carmine, Alexander’s stain

Cite This Article

FRESCURA, V. D., DAIL, H., SCOTTI, T., TEDESCO, S. B. (2012). Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods. BIOCELL, 36(3), 143–145.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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