Table of Content

Single Cell Analysis: Technology and Application

Submission Deadline: 30 March 2022 (closed)

Guest Editors

Prof. Shuhua Yue, Institute of Medical Photonics, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, China.

Prof. Jian Yu, Interdisciplinary Institute for Cancer Diagnosis and Treatment, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, China.


Cellular homeostasis is maintained by a series of chemical transformations that are highly dynamic and tightly regulated in every single cells. Dysregulation in cellular homeostasis leads to many prevalent human diseases. Conventional biochemical assays could demonstrate the presence and concentration of biomolecules extracted from isolated cells or tissue homogenates, however, some important target molecules with small quantities are often buried in the large background of dominant species due to the limited detection sensitivity. Moreover, the investigation of any type of disease mechanism and its progression still remains challenging due to cellular heterogeneity characteristics and physiological state of cells in a given population. Therefore, development of new single cell analysis techniques, including imaging, sensing, manipulating, and sorting, can be beneficial for providing information regarding spatial and temporal dynamics, and elucidating how exactly the biomolecules are metabolized in single live cells. Also, single-cell omics can provide valuable information about functional mutation and copy number variations of cells, which can help to provide a better understanding of intracellular interactions and environmental responses of cellular organelles.


This special issue will focus on the development of single cell analysis techniques and their applications in biological discovery, disease diagnosis and treatment, as well as future prospects regarding health benefits. We invite authors to submit a wide range of manuscripts, including original research, short commentary, and review articles in this area.


Single Cell, Imaging, Sensing, Manipulating, Sorting, Diagnosis, Treatment

Published Papers

  • Open Access


    Synergy of single-cell sequencing analyses and in vivo lineage-tracing approaches: A new opportunity for stem cell biology

    BIOCELL, Vol.46, No.5, pp. 1157-1162, 2022, DOI:10.32604/biocell.2022.018960
    (This article belongs to this Special Issue: Single Cell Analysis: Technology and Application)
    Abstract Single-cell sequencing technologies have rapidly progressed in recent years, and been applied to characterize stem cells in a number of organs. Somatic (postnatal) stem cells are generally identified using combinations of cell surface markers and transcription factors. However, it has been challenging to define micro-heterogeneity within “stem cell” populations, each of which stands at a different level of differentiation. As stem cells become defined at a single-cell level, their differentiation path becomes clearly defined. Here, this viewpoint discusses the potential synergy of single-cell sequencing analyses with in vivo lineage-tracing approaches, with an emphasis on practical considerations in stem cell biology. More >

  • Open Access


    Real-Time analysis of exosome secretion of single cells with single molecule imaging

    BIOCELL, Vol.45, No.6, pp. 1449-1451, 2021, DOI:10.32604/biocell.2021.017607
    (This article belongs to this Special Issue: Single Cell Analysis: Technology and Application)
    Abstract The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity. More >

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