Special lssues
Table of Content

Cell-Based Regenerative Therapies

Submission Deadline: 31 December 2022 (closed)

Guest Editors


Dr. Hamed Nosrati, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran. hamednosratibio@gmail.com


Dr. Mohammad Khodaei, Department of Materials Science and Engineering, Golpayegan University of Technology, Golpayegan, Iran. khodaei@gut.ac.ir

Summary

This issue focuses on the development of therapeutic approaches that combine stem/progenitor cells, growth factors and other bioactive agents with biomaterials and scaffolds to restore, maintain or improve tissue or organ function. Studies on the interactions between cells and constructs are also welcome, particularly where they advance our understanding of these processes or provide clear suggestions for improvements to existing systems. Papers presenting the design and fabrication of scaffolds will only be considered when these have been tested for their ability to support cells proliferation, migration and differentiation. Manuscripts addressing fundamental issues of stem cell biology, namely those related with stem cell plasticity, potency, etc., will be considered only if there is some discussion on potential strategies for their application in cell-based therapeutic approaches. Surface modification technologies and their effects on cell adhesion, proliferation and differentiation are of interest to the journal.

 

Areas of interest include, but are not limited to:

- Stem/progenitor cells in tissue engineering

- Regenerative medicine

- Regenerative pharmacology

- Cell therapy

- Bio-printing

- Surface modification


Keywords

Regenerative Medicine, Stem Cells, Tissue Engineering, Cell Therapy, Cell Culture, Bioreactors, Progenitor Cells, Gene Therapy, Bio-Printing, Surface Modification

Published Papers


  • Open Access

    ARTICLE

    Exosomal miR-30a-5p targets NLRP3 to suppress podocyte pyroptosis in diabetic nephropathy

    WEI LU, KAN GUO, DIANMEI XI, ZHAOXIA XIA
    BIOCELL, Vol.47, No.9, pp. 1995-2008, 2023, DOI:10.32604/biocell.2023.024591
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Background: Mesenchymal stem cell (MSC)-derived exosomes are closely related to pyroptosis in diabetic nephropathy (DN). This study aimed to explore the protective effect of exosomal miR-30a-5p on podocyte pyroptosis in DN. Methods: Streptozotocin was used to establish the mouse model of DN. Human bone marrow MSC-derived exosomes were extracted and identified via transmission electron microscopy, nanoparticle tracking analysis, and western blotting. MiR-30a-5p mimics and non-control (NC) mimics were transfected into MSCs and podocytes, and exosomes were isolated from the MSCs. High glucose (HG)-induced podocyte model was established to determine the effect of exosomal miR-30a-5p on pyroptosis… More >

  • Open Access

    ARTICLE

    The potency of N, N'-diphenyl-1,4-phenylenediamine and adipose-derived stem cell co-administration in alleviating hepatorenal dysfunction complications associated with type 1 diabetes mellitus in rats

    HANY M. ABD EL-LATEEF, SAFA H. QAHL, EMAN FAYAD, SARAH A. ALTALHI, IBRAHIM JAFRI, EL SHAIMAA SHABANA, MARWA K. DARWISH, REHAB MAHER, SAAD SHAABAN, SHADY G. EL-SAWAH
    BIOCELL, Vol.47, No.8, pp. 1885-1895, 2023, DOI:10.32604/biocell.2023.030680
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Background: The increasing occurrence of diabetes mellitus (DM) noted worldwide has considerably elicited concern in the recent past. DM is associated with elevated vascular complications, morbidity, mortality, and poor quality of life. In this context, mesenchymal stem cells (MSCs) have shown significant therapeutic potentialities in managing and curing type 1 DM owing to their self-renewable, immunosuppressive, and differentiation capacities. We investigated the potential action of N, N′-diphenyl-1,4-phenylenediamine (DPPD), a well-known synthetic antioxidant to enhance the therapeutic ability of the adipose-derived stem cells (AD-MSCs) in alleviating kidney and liver complications in diabetic rats. Methods: Over the… More >

    Graphic Abstract

    The potency of <i>N</i>, <i>N'</i>-diphenyl-1,4-phenylenediamine and adipose-derived stem cell co-administration in alleviating hepatorenal dysfunction complications associated with type 1 diabetes mellitus in rats

  • Open Access

    REVIEW

    Anti-fibrotic and anti-inflammatory effect of mesenchymal stromal cell-derived extracellular vesicles in chronic kidney disease

    GIULIA CHIABOTTO, STEFANIA BRUNO
    BIOCELL, Vol.47, No.7, pp. 1499-1508, 2023, DOI:10.32604/biocell.2023.028121
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Renal fibrosis and inflammation are common pathological features of chronic kidney disease (CKD). Since currently available treatments can only delay the progression of CKD, the outcome of patients with CKD is still poor. One therapeutic option for the prevention of CKD-related complications could be the use of mesenchymal stromal cells (MSCs), which have shown beneficial effects in tissue fibrosis and regeneration after damage. However, safety issues, such as cellular rejection and carcinogenicity, limit their clinical application. Among the bioactive factors secreted by MSCs, extracellular vesicles (EVs) have shown the same beneficial effect of MSCs, without More >

    Graphic Abstract

    Anti-fibrotic and anti-inflammatory effect of mesenchymal stromal cell-derived extracellular vesicles in chronic kidney disease

  • Open Access

    REVIEW

    Understanding cell-extracellular matrix interactions for topology-guided tissue regeneration

    AAYUSHI RANDHAWA, SAYAN DEB DUTTA, KEYA GANGULY, TEJAL V. PATIL, RACHMI LUTHFIKASARI, KI-TAEK LIM
    BIOCELL, Vol.47, No.4, pp. 789-808, 2023, DOI:10.32604/biocell.2023.026217
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Tissues are made up of cells and the extracellular matrix (ECM) which surrounds them. These cells and tissues are actively adaptable to enduring significant stress that occurs in daily life. This astonishing mechanical stress develops due to the interaction between the live cells and the non-living ECM. Cells in the matrix microenvironment can sense the signals and forces produced and initiate a signaling cascade that plays a crucial role in the body’s normal functioning and influences various properties of the native cells, including growth, proliferation, and differentiation. However, the matrix’s characteristic features also impact the More >

  • Open Access

    ARTICLE

    Single-cell sequencing analysis reveals the molecular mechanism of promotion of SCAP proliferation upon AZD2858 treatment

    YIFAN XU, DONGMEI CHENG, LEI HU, XIN DONG, LIYING LV, CHEN ZHANG, JIAN ZHOU
    BIOCELL, Vol.47, No.4, pp. 825-836, 2023, DOI:10.32604/biocell.2023.026122
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract The Wnt/β-catenin signaling pathway is the main target of tooth regeneration regulation. Treatment of cells with AZD2858 stimulates the Wnt/β-catenin signaling pathway, yet the function of this pathway in tooth regeneration remains unclear. Here, we found that AZD2858 promotes the accumulation of β-catenin in the nuclei of stem cells from the apical papilla (SCAPs) and enhances cell proliferation. Single-cell sequencing was performed on SCAPs treated with AZD2858. Eight clusters were identified, namely SCAPs-CNTNAP2, SCAPs-DTL, SCAPs-MYH11, SCAPs-MKI67, SCAPs-CXCL8, SCAPs-TPM2, SCAPs-IFIT2 and SCAPs-NEK10. The pseudo-time trajectory analysis showed that AZD2858 enhanced the evolution of SCAPs from SCAPs-TMP2 More >

  • Open Access

    REVIEW

    Interleukin-1 receptor antagonist: From synthesis to therapeutic applications

    KANWAL REHMAN, AMMARA AFZAAL, MUHAMMAD SAJID HAMID AKASH, MUHAMMAD IMRAN, MOHAMMED A. ASSIRI
    BIOCELL, Vol.47, No.4, pp. 809-823, 2023, DOI:10.32604/biocell.2023.025850
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract The cytokine channel’s mechanism for self-regulation involves the application of antagonistic cytokines that are synthesized to connect to the receptors and release soluble cytokine receptors. The very first receptor antagonist of cytokine that was naturally present was interleukin-1 receptor antagonist (IL-1Ra). The IL-1Ra protein forms are disinfected from supernatants of cultured monocytes on stacked IgG. The family of IL-1 consists of IL-1α, IL-1β and IL-1Ra. Human monocytes regulate the production of IL-Ra. IL-Ra takes part in normal physiological functions by using specific antibodies, and acts as an anti-inflammatory agent. IL-Ra is synthesized in the tissues More >

  • Open Access

    REVIEW

    Dental pulp stem cells and banking of teeth as a lifesaving therapeutic vista

    SUKUMARAN ANIL, RAMYA RAMADOSS, NEBU G. THOMAS, JASMIN M. GEORGE, VISHNUPRIYA K. SWEETY
    BIOCELL, Vol.47, No.1, pp. 71-80, 2023, DOI:10.32604/biocell.2023.024334
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest, process, and cryogenically preserve dental pulp stem cells. Future stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem cells. Teeth serve as a substantial source of mesenchymal stem cells, otherwise disposed of as medical waste. Care should be taken to store this treasure trove of stem cells. Collective responsibility of patients, dentists, and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the More >

  • Open Access

    ARTICLE

    Microenvironmental regulation of stem cells injected in the area at risk of neurodegenerative diseases

    JU HYUNG LEE, IL-KWON KIM, SANG WOO KIM, SOYEON LIM, SEAHYOUNG LEE, KI-CHUL HWANG, BYEONG-WOOK SONG
    BIOCELL, Vol.46, No.10, pp. 2231-2234, 2022, DOI:10.32604/biocell.2022.020179
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract The complex mechanism of degenerative diseases and the non-specific modulation of regenerative targets are topics that need to be elucidated in order to advance the use of stem cells in improvement of neurodegenerative diseases. From pre-transplantation through post-transplantation, there are many changes in the conditions, both inside and outside of the stem cells that have not been carefully considered. This has hindered development in the field of cell therapy and regeneration. This viewpoint highlights the potential implications of intracellular and extracellular alterations of stem cells in transplanted areas at risk of neurodegenerative disease. More >

  • Open Access

    ARTICLE

    Calcitonin gene related peptide modified mesenchymal stem cells reduce restenosis after carotid balloon injury in rats

    PANKE CHEN, SHUAI MA
    BIOCELL, Vol.46, No.8, pp. 1903-1909, 2022, DOI:10.32604/biocell.2022.018651
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract This work aimed to investigate the effects of calcitonin gene-related peptide (CGRP)-modified mesenchymal stem cells (MSCs) on vascular stenosis in carotid balloon-injured rats. The CGRP gene labeled with EGFP was transfected into bone marrow MSCs, and CGRP protein expression in MSCs was confirmed by immunofluorescence assays. A rat carotid balloon injury model was established using a surgical method. CGRP-modified MSCs were orthotopically transplanted into the injured area of the rats. At 28 days after cell transplantation, EGFP and CD31 expression was detected by immunofluorescence staining. Hematoxylin-eosin (H&E) staining was used to detect the intima/media area… More >

  • Open Access

    REVIEW

    The role of transcriptional factor brachyury in the development and repair of nucleus pulposus

    YINGHUI WU, HONG ZHANG, QIANG WANG, SUOYUAN LI, JUN SHEN
    BIOCELL, Vol.46, No.6, pp. 1363-1364, 2022, DOI:10.32604/biocell.2022.018360
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Transcription factor Brachyury, a protein containing 435 amino acids, has been widely investigated and reported in notochord differentiation and nucleus pulposus development. The crucial functions and underlying mechanisms by Brachyury are discussed in this paper, which suggests Brachyury can be developed into a potential novel target for the therapy of intervertebral disc degeneration. More >

  • Open Access

    ARTICLE

    Transplanted choroidal plexus epithelial cells can integrate with organotypic spinal cord slices into a new system

    JINGJIE LIU, XIAOYAN DING, LI XIANG, SHENGLI HUANG
    BIOCELL, Vol.46, No.6, pp. 1537-1544, 2022, DOI:10.32604/biocell.2022.018441
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract This study aimed to evaluate the integration of transplanted choroidal plexus epithelial cells with organotypic spinal cord slices. Organotypic spinal cord slices, normally cultured for 6 days, were divided into control group (Ctrl) and transplanted group (T). The choroidal plexus epithelial cells were dissociated and primary cultured (C group). The choroidal plexus epithelial cells cultured for 6–7 days were labeled by 1,1’-dioctadecyl-3,3,3’,3’-tetramethyl-indocarbocyanineperchlorate (CM-Dil), and were identified by transthyretin (TTR) in immunocytochemistry. They were adjusted to the density of 0.5–1 × 107/ml, then 2 μl cells suspension were transplanted to the spinal cord slices in the T… More >

  • Open Access

    ARTICLE

    Salubrinal alleviates traumatic spinal cord injury through suppression of the eIF2α/ATF4 pathway in mouse model

    XINFU FAN, JIE CHEN, ZONGMENG ZHANG, FANGHUI CHEN, HAOSEN WANG, YAFEI CAI, JUN LI
    BIOCELL, Vol.46, No.6, pp. 1527-1535, 2022, DOI:10.32604/biocell.2022.018269
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Spinal cord injury (SCI) remains an intractable clinical challenge of neurosurgery, it can be divided into two stages: uncontrollable primary injury induced by mechanical damage and controllable secondary injury regulated by continuous cell death. The apoptosis was the one of most important events in secondary injury, previous studies revealed that excessive endoplasmic reticulum (ER) stress breaks down the homeostasis and triggers apoptosis in the spinal cord. To deter or alleviate the secondary jury, we screen one of fat-soluble compounds, salubrinal, which was an inhibitor of eIF2α dephosphorylation can repair SCI by inhibiting ER stress in… More >

  • Open Access

    REVIEW

    Mesenchymal stem cells: As a multi-target cell therapy for clearing β-amyloid deposition in Alzheimer’s disease

    RUXIN ZHANG, CHENGGANG LI, RUOCHEN DU, YITONG YUAN, BICHUN ZHAO, YUJUAN ZHANG, CHUNFANG WANG
    BIOCELL, Vol.46, No.3, pp. 583-592, 2022, DOI:10.32604/biocell.2022.017248
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Extracellular β-amyloid (Aβ) plaques and neurofibrillary tangles (NFTs) are the pathological hallmarks of Alzheimer’s disease (AD). Studies have shown that aggregates of extracellular Aβ can induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors. Thus, modulation of Aβ might be a potential therapeutic strategy for modifying disease progression. Recently, a large number of reports have confirmed the beneficial effects of mesenchymal stem cells (MSCs) on AD. It is believed to reduce neuroinflammation, reduce Aβ amyloid deposits and NFTs, increase acetylcholine levels, promote neurogenesis, reduce neuronal damage, and improve working memory and More >

  • Open Access

    ARTICLE

    Anti-inflammatory and antioxidant potential capacities of AD-MSCs and BM-MSCs in suppressing pancreatic β-cells auto-immunity and apoptosis in rats with T1DM induced model

    SHADY G. EL-SAWAH, FAYEZ ALTHOBAITI, HANAN M. RASHWAN, ADIL ALDHAHRANI, MARWA A. ABDEL-DAYEM, EMAN FAYAD, REHAB M. AMEN, EL SHAIMAA SHABANA, EHAB I. EL-HALLOUS
    BIOCELL, Vol.46, No.3, pp. 745-757, 2022, DOI:10.32604/biocell.2022.017853
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Since Type 1 diabetes (T1DM) occurs when β-cells mass is reduced to less than 20% of the normal level due to autoimmune destruction of cells resulting in the inability to secrete insulin, preservation or replenishment of the functional β-cells mass has become a major therapeutic focus for this diabetic type treatment. Thus, this 4-week work plan was designed to determine which mesenchymal stem cells (MSCs) type is more appropriate to alleviate pancreatic hazards resulting from diabetes induction; via tracking a comparative study between MSCs derived from adipose tissue (AD-MSCs) and from bone marrow (BM-MSCs) in… More >

  • Open Access

    REVIEW

    A review from mesenchymal stem-cells and their small extracellular vesicles in tissue engineering

    ODIN RAMIREZ-FERNANDEZ, ESMERALDA ZUÑIGA-AGUILAR
    BIOCELL, Vol.46, No.2, pp. 325-338, 2022, DOI:10.32604/biocell.2022.016892
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract This review aims to offer a vision of the clinical reality of cell therapy today in intensive medicine. For this, it has been carried out a description of the properties, functions, and Mesenchymal Stem Cells (MSCS) sources to subsequently address the evidence in preclinical models and studies clinical trials with whole cells and models attributed to small extracellular vesicles (sEVs), nanoparticles made up of microvesicles secreted by cells with an effect on the extracellular matrix, and their impact as an alternative towards cell-free regenerative medicine. MSCs are cells that enhance the regenerative capacity which can… More >

  • Open Access

    ARTICLE

    Investigation of the antioxidant defensive role of both AD-MSCs and BM-MSCs in modulating the alteration in the oxidative stress status in various STZ-diabetic rats’ tissues

    SHADY G. EL-SAWAH, FAYEZ ALTHOBAITI, ADIL ALDHAHRANI, EMAN FAYAD, MARWA A. ABDEL-DAYEM, REHAB M. AMEN, EL SHAIMAA SHABANA, EHAB I. EL-HALLOUS, HANAN M. RASHWAN
    BIOCELL, Vol.45, No.6, pp. 1561-1568, 2021, DOI:10.32604/biocell.2021.016869
    (This article belongs to the Special Issue: Cell-Based Regenerative Therapies)
    Abstract Diabetes mellitus (DM) could negatively affect patients’ health via inducing a lot of serious functional hazards in many tissues’ cells at molecular levels. Recently, many scientists had proposed stem cell therapy being an appropriate alternative treatment protocol for numerous health threatening issues including diabetes. Therefore, the current study was designed to investigate the antioxidant potentiality of two MSCs types in alleviating tissues’ oxidative stress dramatic elevation resulting as a consequence of Type 1 DM induction. In our 4 weeks study, animals were divided into four groups: control group, STZ-diabetic group (D), D+AD-MSCs group and D+BM-MSCs… More >

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