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A global study of transcriptome dynamics in the venom gland of Solenopsis invicta Buren during laboratory rearing

LIMIN CHEN1,2, XIAOWEI LI1, TIANJUN HE2, QUANCONG WU2, TINGTING CHEN3, JUN HUANG1,*, YAOBIN LU1,*
1 State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China
2 Integrated Plant Protection Center, Lishui Academy of Agricultural and Forestry Sciences, Lishui, 323000, China
3 College of Ecology, Lishui University, Lishui, 323000, China
* Address correspondence to: Yaobin Lu, luybcn@163.com; Jun Huang, junhuang1981@aliyun.com
* Corresponding Authors:YAOBIN LU. Email: ; JUN HUANG. Email:

BIOCELL 2022, 46(3), 821-828. https://doi.org/10.32604/biocell.2021.016450

Received 07 March 2021; Accepted 09 April 2021; Issue published 18 November 2021

Abstract

Venom plays several important roles in the life of the fire ant, Solenopsis invicta Buren. Laboratory rearing significantly affected the toxin component of S. invicta. However, the molecular mechanism of the change of venomous components when kept in the laboratory is unclear. In this study, RNA sequencing technique (RNA-Seq) was performed to explore differentially expressed genes in the venom gland of S. invicta at 0, 10, and 60 days after laboratory rearing. The RNA-Seq results showed that the expression of a large number of genes changed. The DEGs were involved in multiple pathways, including proteolysis, serine-type endopeptidase, and allergen. Furthermore, RNA-Seq and qRT-PCR data revealed that the expression of some genes related to proteolysis and allergen significantly decreased. Thus, our data generated new data relating to toxin-component and the transcriptome dynamics in the venom gland of S. invicta during laboratory rearing.

Keywords

Solenopsis invicta Buren, RNA-Seq, Venom, Laboratory rearing

Cite This Article

Chen, L., Li, X., He, T., Wu, Q., Chen, T. et al. (2022). A global study of transcriptome dynamics in the venom gland of Solenopsis invicta Buren during laboratory rearing. BIOCELL, 46(3), 821–828.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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