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Ketamine effect on intracellular and mitochondrial calcium mobilization

Juanita BUSTAMANTE1,*, Analía CZERNICZYNIEC2, Silvia LORES-ARNAIZ2

1 Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS), Facultad de Medicina, Universidad Abierta Interamericana, Buenos Aires, Argentina
2 Instituto de Bioquímica y Medicina Molecular, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina

* Address correspondence to: Juanita Bustamante,

BIOCELL 2016, 40(1), 11-14. https://doi.org/10.32604/biocell.2016.40.011

Abstract

The suppressive effects of ketamine on intracellular calcium has been reported in a variety of cells although the mechanisms involved are not well understood. The aim of this work was to evaluate the ketamine effect on the mitochondrial Ca2+ accumulation and the cellular Ca2+ mobilization using FLUO4-AM and flow cytometry. The results showed that mitochondria from ketamine injected animals presented a lower ability to retain calcium at concentrations higher than 20 μM, as compared with controls (saline injected animals). In addition, ketamine showed a significant decreased KCl-induced intracellular calcium concentration. KCl increased calcium influx through cellular depolarization. According to the data presented herein, ketamine presents a clear inhibitory effect on cytosolic Ca2+ transport mechanisms, independently from their action on the calcium channel associated NMDA receptor.

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BUSTAMANTE, J., CZERNICZYNIEC, A., LORES-ARNAIZ, S. (2016). Ketamine effect on intracellular and mitochondrial calcium mobilization. BIOCELL, 40(1), 11–14.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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