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LINC00609 inhibits A549 cells progression through the regulation of miR-128-3p/RND3 axis

XIANGCHAO DING1,#, YANG ZHAO2,#, XINGHUA ZHANG1, HUIQING LIN1,*

1 Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China
2 Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China

* Corresponding Author: HUIQING LIN. Email: email
# Co-first authors: Xiangchao Ding, Yang Zhao

BIOCELL 2023, 47(5), 1117-1126. https://doi.org/10.32604/biocell.2023.026715

Abstract

Background: Long-chain non-coding RNA (lncRNA) LINC00609 is a potential tumor suppressor, but the mechanism of action in non-small cell lung cancer (NSCLC) is yet to be understood.Objectives: The effects of LINC00609 on A549 cell proliferation, apoptosis, and cell cycle arrest were investigated. Methods: The LINC00609 levels in NSCLC and normal tissues were analyzed by bioinformatics. Expressions of LINC00609, miR-128-3p, and Rho family GTPase 3 (RND3) in NSCLC cells (A549) were determined by qRT-PCR. Bioinformatics analysis predicted target genes and dual-luciferase reporter assays to ensure that LINC00609 targeted miR-128-3p and miR-128-3p targeted RND3. The proliferation of cells was determined using EDU and CCK-8. Flow cytometry was used to evaluate cell apoptosis rate and cell cycle. The western blotting assay identified proteins related to proliferation and apoptosis. Results: In NSCLC tissues, LINC00609 was expressed in low levels, while its high expression was associated with a higher survival rate. LINC00609 affected cell proliferation, apoptosis, cell cycle arrest, and expression of related proteins. Dual-luciferase reporter assay showed that LINC00609 binds specifically to miR-128-3p, and miR-128-3p binds to RND3. MiR-128-3p overexpression could neutralize the effects of LINC00609. A siRNA targeting RND3 could reverse the effect of the miR-128-3p inhibitor. Silencing RND3 resulted in a decrease in apoptosis rate and the number of cells in the S-phase and an increase in the number of cells in the G1-phase. Furthermore, phosphorylation levels of the AKT protein and mTOR protein, and Bcl2 expression, increased; however, the expression of RND3, Bax, and caspase3 decreased. Conclusions: LINC00609 regulated miR-128-3p/RND3 axis to modulate A549 cell proliferation, apoptosis, and cell cycle arrest. In the case of NSCLC, LINC00609 could be a potential target for therapy.

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APA Style
DING, X., ZHAO, Y., ZHANG, X., LIN, H. (2023). linc00609 inhibits A549 cells progression through the regulation of mir-128-3p/rnd3 axis. BIOCELL, 47(5), 1117-1126. https://doi.org/10.32604/biocell.2023.026715
Vancouver Style
DING X, ZHAO Y, ZHANG X, LIN H. linc00609 inhibits A549 cells progression through the regulation of mir-128-3p/rnd3 axis. BIOCELL . 2023;47(5):1117-1126 https://doi.org/10.32604/biocell.2023.026715
IEEE Style
X. DING, Y. ZHAO, X. ZHANG, and H. LIN "LINC00609 inhibits A549 cells progression through the regulation of miR-128-3p/RND3 axis," BIOCELL , vol. 47, no. 5, pp. 1117-1126. 2023. https://doi.org/10.32604/biocell.2023.026715



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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