Open Access
ARTICLE
Upregulation of histone H3 caused by CRYAA may contribute to the development of age-related cataract
CHAO WANG1,2, JUNWEI WANG1, FANQIAN SONG1,2, HANRUO LIU3, LIYAO SUN1,2, XI WEI1,2, TAO ZHENG1, HUA QIAN2, XIAOGUANG LI2, WEIHUA ZHANG4, XIANLING TANG1,*, PING LIU1,*
1 Eye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, 150001, China
2 Department of Pharmacology, College of Pharmacy, Harbin Medical University, and Heilongjiang Academy of Medical Sciences, Harbin, 150081, China
3 Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Lab, Beijing, 100000, China
4 Department of Pathophysiology, Harbin Medical University, Harbin, 150081, China
* Corresponding Authors: Ping Liu, ; Xianling Tang,
(This article belongs to the Special Issue: )
BIOCELL 2023, 47(1), 143-154. https://doi.org/10.32604/biocell.2023.023585
Received 04 May 2022; Accepted 12 July 2022; Issue published 26 September 2022
Abstract
Objective: Age-relate cataract (ARC) is a disease of the eyes with no effective drugs to prevent or treat patients.
The aim of the present study is to determine whether histone H3, αA-crystallin (CRYAA), β-galactosidase (GLB1), and
p53 are involved in the pathogenesis of ARC.
Methods: A total of 99 anterior lens capsules (ALCs) of patients with ARC
of various nuclear grades, ultraviolet models of ALCs, and two human lens epithelial cell lines (FHL-124 and SRA01/04)
were used, and the expression of histone H3, CRYAA, GLB1, and p53 were detected by immunoblotting and reverse
transcription and real time-quantitative polymerase chain reaction. The association between CRYAA with histone H3,
GLB1, and p53 was assessed in FHL-124 and SRA01/04 cells following CRYAA overexpression.
Results: Histone H3
and p53 in ALCs of patients with ARC were up-regulated in a grade-dependent manner, and the expression of
CRYAA showed a positive association with histone H3, p53, and GLB1. In UV models of ALCs and human lens
epithelial cell lines, the expression levels of histone H3, cell apoptosis factors (Bax/Bcl-2, cleaved caspase-3), and
inflammation factors (interleukin-6, tumor necrosis factor-α) were all up-regulated. Furthermore, transfection of
CRYAA in FHL-124 cells induced overexpression of histone H3.
Conclusion: CRYAA-mediated upregulation of
histone H3 may be involved in the pathogenesis of ARC. p53 may also have a role in ARC development, but not via
the CRYAA-histone H3 axis. The results of the present study may assist in improving our understanding of the
pathogenesis of ARC and in identifying potential targets for treatment.
Keywords
Cite This Article
WANG, C., WANG, J., SONG, F., LIU, H., SUN, L. et al. (2023). Upregulation of histone H3 caused by CRYAA may contribute to the development of age-related cataract.
BIOCELL, 47(1), 143–154. https://doi.org/10.32604/biocell.2023.023585