Vol.44, No.2, 2020, pp.193-199, doi:10.32604/biocell.2020.08648
Effect of Black Sea bream extracts on cytokine production in lipopolysaccharide-induced inflammation
1 Ocean Science and Technology School, Korea Maritime & Ocean University, Busan, Korea
2 Department of Microbiology, Kosin University College of Medicine, Busan, Korea
3 Division of Marine Bioscience, Korea Maritime & Ocean University, Busan, Korea
* Address correspondence to: Sunyoung Lim, sylim@kmou.ac.kr
Received 19 September 2019; Accepted 16 December 2019; Issue published 27 May 2020
We investigated the effect of black sea bream extracts on changes in pro-inflammatory and anti-inflammatory cytokines induced by lipopolysaccharide (LPS) in murine splenocytes. The levels of pro-inflammatory cytokines [interleukin (IL)-6, tumor necrosis factor-α (TNF-α), IL-12/IL-23(p40) and IL-17A] and anti-inflammatory cytokines [IL-4, IL-10 and interferon-γ (IFN-γ)] were assessed. Incubation of murine splenocytes with acetone+methylene chloride (A+M) and methanol (MeOH) extracts significantly decreased LPS-induced IL-6, IL-12/IL-23(p40) and IL-17A productions after 6 h incubation (p < 0.05). The A+M and MeOH extracts significantly increased LPS-induced IL-4 and IFN-γ productions at 48 and 72 h incubation (p < 0.05). Treatment with A+M extract resulted in significantly higher IL-10 production in splenocytes after 72 h (p < 0.05). Conclusively, black sea bream extracts were shown to be efficient in falling several pro-inflammatory cytokines while rising anti-inflammatory cytokines. Thus our results suggest that black sea bream extracts selectivity modulate immune events.
Black Sea bream, Cytokines, Interleukin-4, Interleukin-6, Interleukin-17A
Cite This Article
NA, E., CHOI, M., PARK, I., LIM, S. (2020). Effect of Black Sea bream extracts on cytokine production in lipopolysaccharide-induced inflammation. BIOCELL, 44(2), 193–199.
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