Open Access

ARTICLE

Oncolytic adenovirus targeting LASP-1 inhibited renal cell cancer progression

FANGHAO SUN¹, WENCHAO ZHAO¹, LIANSHENG ZHANG², HONGGUI MA¹, JIAN ZHOU¹, YOUGAN CHEN¹, JIANQUAN HOU^1,*
1 Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China
2 Department of Urology, Soochow University Affiliated Wuxi Ninth Hospital, Wuxi, 214000, China
* Address correspondence to: Jianquan Hou,

BIOCELL 2020, 44(4), 639-647. https://doi.org/10.32604/biocell.2020.013053

Received 23 July 2020; Accepted 24 August 2020; Issue published 24 December 2020

Abstract

Recent studies suggested that LIM and SH3 protein 1 (LASP-1) is a promising therapeutic target for renal cell cancer (RCC). This study aimed to explore the role of LASP-1 in RCC. For this purpose, LASP-1 expression in RCC tissues was analyzed by immunohistochemistry and Western blot analysis. Cell proliferation, migration, invasion, and gene expression were detected by CCK-8 assay, Transwell assay, and Western blot analysis. The results showed that LASP-1 was highly expressed in RCC, and its expression level,t was positively correlated with lymph node metastasis and tumor, nodes, and metastases (TNM) stage. The knockdown of LASP-1 expression significantly inhibited the proliferation of RCC cells, increased the apoptosis rate, and inhibited RCC cell invasion and migration by inhibiting epithelial–mesenchymal transition. We conclude that LASP-1 promotes RCC progression and metastasis and is a promising therapeutic target for RCC.

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Keywords

LASP-1, Oncolytic adenovirus, Renal cell cancer

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