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Exosomes derived from osteoclasts under compression stress inhibit osteoblast differentiation

YUE WANG, YUNFEI ZHENG*, WEIRAN LI*

Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, 10081, China

* Address correspondence to: Yunfei Zheng, email; Weiran Li, email

BIOCELL 2021, 45(2), 427-444. https://doi.org/10.32604/biocell.2021.013960

Abstract

Orthodontic tooth movement is triggered by orthodontic force loading on the periodontal ligament and is achieved by alveolar bone remodeling, which is regulated by intimate crosstalk between osteoclastogenesis and osteoblast differentiation. Whether the communication between osteoclasts and osteoblasts is influenced by orthodontic compression stress requires further clarification. In this study, osteoclasts were differentiated for 10 days. On day 4 of differentiation, the number of pre-osteoclasts peaked, as determined by the increased expression of RANK and the number of multinucleated cells. After 24 h of compression stress loading, on day 4, the number of osteoclasts increased, and the optimal magnitude of stress to promote osteoclastogenesis was determined as 1 g/cm2. Moreover, the results of RNA-sequencing analysis showed that the miRNA expression profile changed markedly after compression loading and that many of the altered miRNAs were associated with cell communication functions. A series of indirect co-culture experiments showed an inhibitory effect of osteoclasts on osteoblast differentiation, especially after compression. Next, we added osteoclast-derived exosomes to hPDLSCs during osteoblast differentiation. Exosomes derived from osteoclasts under compression (cEXO) showed a greater inhibitory effect on osteoblast differentiation, compared to exosomes from osteoclasts without compression (EXO). Therefore, we analyzed differentially expressed miRNAs associated with bone development functions in exosomes: miR-223-5p and miR-181a-5p were downregulated, whereas miR-133a-3p, miR-203a-3p, miR-106a-5p, and miR-331-3p were upregulated; these altered expressions may explain the enhanced inhibitory effect of compression stress.

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APA Style
WANG, Y., ZHENG, Y., LI, W. (2021). Exosomes derived from osteoclasts under compression stress inhibit osteoblast differentiation. BIOCELL, 45(2), 427-444. https://doi.org/10.32604/biocell.2021.013960
Vancouver Style
WANG Y, ZHENG Y, LI W. Exosomes derived from osteoclasts under compression stress inhibit osteoblast differentiation. BIOCELL . 2021;45(2):427-444 https://doi.org/10.32604/biocell.2021.013960
IEEE Style
Y. WANG, Y. ZHENG, and W. LI "Exosomes derived from osteoclasts under compression stress inhibit osteoblast differentiation," BIOCELL , vol. 45, no. 2, pp. 427-444. 2021. https://doi.org/10.32604/biocell.2021.013960

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cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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