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HSA-MIR-203/MyD88 axis mediates the protective effect of hispidulin on LPS-induced apoptosis in a human renal tubular epithelial line, HK-2

SICONG WANG1, RUIJIN LIU2, QIUYUAN HAN2, KAIJIANG YU3,*
1 Department of Critical Care Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150001, China
2 Department of Critical Care Medicine, The Cancer Hospital of Harbin Medical University, Harbin, 150081, China
3 Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China
* Corresponding Author:KAIJIANG YU. Email:

BIOCELL 2022, 46(1), 149-158. https://doi.org/10.32604/biocell.2021.013027

Received 22 July 2020; Accepted 21 December 2020; Issue published 29 September 2021

Abstract

Acute kidney injury (AKI), commonly occurring as complications of sepsis, cardiac surgery, and liver or kidney transplantation, is a critical care syndrome. It is well known that lipopolysaccharide (LPS) shock is a common triggering factor for AKI. This study is aimed to examine the effect of flavonoid compound hispidulin on LPS-induced AKI. For this, renal tubular epithelial cell HK-2 was treated with LPS to establish an in vitro model of AKI. The effect of hispidulin on HK-2 cell viability was examined using CCK-8 assay. Cell apoptosis was determined by TUNEL and flow cytometry. Apoptosis marker proteins were determined by using western blot. The levels of pro-inflammatory cytokines were determined by ELISA assay and qRT-PCR. The translocation of NF-κB was determined by western blot. The effect of MyD88 on the cytoprotective activities of hispidulin was examined by overexpressing MyD88 in HK-2 cells. Our results showed that hispidulin was not able to produce a cytotoxic effect on HK-2 cells at tested concentrations. However, hispidulin could protect HK-2 cells from LPS-induced cell injury. Our results also showed that hispidulin was able to attenuate LPS-induced HK-2 cell apoptosis. In addition, LPS led to an inflammatory response in HK-2 cells, evidenced by NF-κB p65 activation as well as increased expression and release of inflammatory cytokine IL-6 and TNF- α, which could be reversed by pretreatment with hispidulin. Overexpression MyD88 was found to significantly dampen the cytoprotective activities of hispidulin against LPS insult. More importantly, MyD88 was identified as a direct target of hsa-miR-203, and hispidulin was found to regulate the expression of MyD88 via upregulating hsa-miR-203. Our results showed that hispidulin attenuates LPS-induced HK-2 damage via regulating hsa-miR-203/MyD88 axis.

Keywords

Hispidulin, LPS, HK-2, hsa-miR-203, MyD88

Cite This Article

WANG, S., LIU, R., HAN, Q., YU, K. (2022). HSA-MIR-203/MyD88 axis mediates the protective effect of hispidulin on LPS-induced apoptosis in a human renal tubular epithelial line, HK-2. BIOCELL, 46(1), 149–158.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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