Open Access
ARTICLE
Identification of tumorigenic risk genes in human placenta-derived mesenchymal stem cells treated with 3-methylcholanthrene
YUANYUAN JIA, XIAONA MA, XIURUI YAN, JING XUE, TINGTING YANG, XUEYUN LIANG*, XIAOMING LIU*
Institute of Human Stem Cell Research, General Hospital of Ningxia Medical University, Yinchuan, 750004, China
* Corresponding Authors: XUEYUN LIANG, ; XIAOMING LIU,
BIOCELL 2022, 46(2), 479-493. https://doi.org/10.32604/biocell.2022.015390
Received 17 December 2020; Accepted 04 March 2021; Issue published 20 October 2021
Abstract
Mesenchymal stem cells (MSCs) capable of tumour topotaxis have been served as cellular vehicles to deliver
anti-tumour agents. As cellular components of the tumour microenvironment, MSCs also affect tumour progression.
However, the tumour transformation-related genes of MSCs remain unclear since either tumorigenic or tumour
suppressor effects within these cells have been researched. Hence, we aimed to identify potential biomarkers indicative
of tumorigenic risk by RNA-seq analysis of human placenta tissue-derived MSCs (hPTMSCs) exposed to the
carcinogenic agent, 3-methylcholanthrene (3-MC). Twenty-nine tumour transformation-related genes and three
pluripotency-related genes were appraised as differentially expressed genes (DEGs) in hPTMSCs. Overexpression of
sfrp1 led to reduced cell viability, migration, and colony formation in A549. In contrast, the overexpression of
ptgs2
exerted the opposite effect. These results indicate that A549 cells with high
ptgs2 expression but low
sfrp1 expression
may have a more potential tumorigenic capacity. Taken together, this study suggests that
ptgs2 and
sfrp1 may be
tumorigenic risk genes.
Keywords
Cite This Article
JIA, Y., MA, X., YAN, X., XUE, J., YANG, T. et al. (2022). Identification of tumorigenic risk genes in human placenta-derived mesenchymal stem cells treated with 3-methylcholanthrene.
BIOCELL, 46(2), 479–493. https://doi.org/10.32604/biocell.2022.015390