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Ang-(1-7) exerts anti-inflammatory and antioxidant activities on high glucose-induced injury by prohibiting NF-κB-IL-1β and activating HO-1 pathways in HUVECs

FEI CHENG1,#, YIQIAN DING2,#, QING XU3, WEI ZHANG3, YULAN ZHEN4, JING LIU5, SHICHENG LI1, CHANG TU1, GUOHUA LAI1, JUN LAN1,*, JINGFU CHEN1,*

1 Department of Cardiovascular Medicine, Dongguan Songshan Lake Center Hospital, Dongguan Cardiovascular Institute, Dongguan Shilong People’s Hospital Affiliated to Southern Medical University, Dongguan, 523326, China
2 Department of Ultrasound, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
3 Department of Cardiology, Huangpu Division of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, China
4 Department of Oncology, Dongguan Third People’s Hospital, Dongguan, 523326, China
5 Second Ward of Internal Medicine, Dongguan Eighth People’s Hospital, Dongguan Children’s Hospital, Dongguan, 523003, China
# These authors contributed equally to this work

* Corresponding Authors:JUN LAN. Email: email; JINGFU CHEN. Email: email

BIOCELL 2022, 46(4), 1053-1066. https://doi.org/10.32604/biocell.2021.012901

Abstract

Previous reports have suggested that Ang-(1-7) may have a protective effect in endothelial cells against high glucose (HG)-induced cell injury thanks to a modulatory mechanism in the NF-κB signaling pathway. In this study, we have examined whether NF-κB-IL-1β and Heme oxygenase-1 (HO-1) pathways contribute to the protection of Ang-(1-7) against hyperglycemia-induced inflammation and oxidative stress in human umbilical vein endothelial cells (HUVECs). Our results indicate that time-varying exposures of HUVECs, from 1 h to 24 h, to high glucose concentrations result in an increased expression of phosphorylated (p)-p65 and HO-1 in a time-dependent manner. As an inhibitor of NF-κB, pyrrolidinedithiocarbamic acid (PDTC) suppressed IL-1β production induced by HG. Of note, HUVECs previously treated with Ang-(1-7) (2 μM) for 30 min before being exposed to HG concentrations significantly ameliorated the HG-increased in p-p65 and IL-1β expression; whereas obviously up-regulated the level of HO-1, along with inhibition of oxidative stress, inflammation, and the HG-induced cytotoxicity. Importantly, when HUVECs were previously treated either with PDTC or IL-1Ra for 30 min before being exposed to HG, it significantly prevented damages caused by high glucose concentrations mentioned above, while the treatment of HO-1 inhibitor Sn-protoporphyrin (SnPP) before exposure to both HG and Ang-(1-7) significantly blocked the protective effect exerted by Ang-(1-7) on endothelial cells against injuries induced by HG mentioned above. To conclude, the data of this study showed that activation and inhibition of the NF-κB-IL-1β pathway and HO-1 pathway may constitute an important defense mechanism against endothelial cell damage caused by HG concentrations. We additionally gave new evidence showing that exogenous Ang-(1-7) exerts a protective effect on HUVECs against the HG-induced cell injury via the inhibition and the activation of the NF-κB-IL-1β pathway and the HO-1 pathway, respectively.

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CHENG, F., DING, Y., XU, Q., ZHANG, W., ZHEN, Y. et al. (2022). Ang-(1-7) exerts anti-inflammatory and antioxidant activities on high glucose-induced injury by prohibiting NF-κB-IL-1β and activating HO-1 pathways in HUVECs. BIOCELL, 46(4), 1053–1066.



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