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Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes

KAN OUYANG1,2,#, MEIQUAN XU3,#, YUJIE LIANG4, XIAO XU2, LIMEI XU2, CAINING WEN1,2, ZHUAN QIN2, YIXIN XIE2, HUAWEI ZHANG5, LI DUAN2,*, DAPING WANG1,2,5,*

1 Guangzhou Medical University, Guangzhou, 510095, China
2 Department of Sports Medicine, Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, 518035, China
3 Deparment of Pathology, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, 518035, China
4 Shenzhen Key Laboratory for Psychological Healthcare, Shenzhen Institute of Mental Health, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, 518020, China
5 Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China

* Address correspondence to: Li Duan, email; Daping Wang, email
# These authors contributed equally to this work

BIOCELL 2022, 46(6), 1521-1526. https://doi.org/10.32604/biocell.2022.018788

Abstract

There is no efficient tracking system available for the therapeutic molecules delivered to cartilage. The dense matrix covering the cartilage surface is the main biological barrier that the therapeutic molecules must overcome. In this study, we aimed to establish a system that can dynamically and effectively track the therapeutic molecules delivered to cartilage. To this aim, we adopted bovine and human cartilage explants as ex vivo models for chondrocyte-targeted exosome dispersion. The efficiency of drug delivery was evaluated using frozen sections. The results of this study showed that the penetration and distribution of chondrocyte-targeted exosomes in cartilage explants can be tracked dynamically. Thus, ex vivo cartilage explants provide an effective and economic system to evaluate therapeutic drugs encapsulated in chondrocyte-targeted exosomes in preclinical studies.

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APA Style
OUYANG, K., XU, M., LIANG, Y., XU, X., XU, L. et al. (2022). ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes. BIOCELL, 46(6), 1521-1526. https://doi.org/10.32604/biocell.2022.018788
Vancouver Style
OUYANG K, XU M, LIANG Y, XU X, XU L, WEN C, et al. ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes. BIOCELL . 2022;46(6):1521-1526 https://doi.org/10.32604/biocell.2022.018788
IEEE Style
K. OUYANG et al., "Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes," BIOCELL , vol. 46, no. 6, pp. 1521-1526. 2022. https://doi.org/10.32604/biocell.2022.018788



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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