Open Access

ARTICLE

miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome

NINGYU LI^1,2,#, XIAOFANG CHEN^2,#,§, SUXIA GENG², PEILONG LAI², LISI HUANG², MINMING LI², XIN HUANG², CHENGXIN DENG², YULIAN WANG², JIANYU WENG², XIN DU^1,2,*

1 School of Medicine, South China University of Technology, Guangzhou, 510006, China
2 Department of Hematology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China

* Corresponding Authors: Xin Du, ; Jianyu Weng,
# These authors contributed equally to this work
§ Present address: Department of Hematology, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, 510180, China

(This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)

BIOCELL 2023, 47(1), 133-141. https://doi.org/10.32604/biocell.2022.022021

Received 17 March 2022; Accepted 24 May 2022; Issue published 26 September 2022

Abstract

The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis.

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Keywords

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