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miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome

NINGYU LI1,2,#, XIAOFANG CHEN2,#,§, SUXIA GENG2, PEILONG LAI2, LISI HUANG2, MINMING LI2, XIN HUANG2, CHENGXIN DENG2, YULIAN WANG2, JIANYU WENG2, XIN DU1,2,*

1 School of Medicine, South China University of Technology, Guangzhou, 510006, China
2 Department of Hematology, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, 510080, China

* Corresponding Authors: Xin Du, email; Jianyu Weng, email
# These authors contributed equally to this work
§ Present address: Department of Hematology, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, 510180, China

(This article belongs to this Special Issue: Decoding Gene (including circRNA, lincRNA miRNA and mRNA) Expression)

BIOCELL 2023, 47(1), 133-141. https://doi.org/10.32604/biocell.2022.022021

Abstract

The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis.

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Cite This Article

LI, N., CHEN, X., GENG, S., LAI, P., HUANG, L. et al. (2023). miR-103-3p regulates the differentiation of bone marrow mesenchymal stem cells in myelodysplastic syndrome. BIOCELL, 47(1), 133–141.



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