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Increased MAD2L2 expression predicts poor clinical outcome in Colon Adenocarcinoma

HAOTONG SUN1,2, HEYING WANG1,3, XIN LI1,2, YANJIE HAO1,2, JUN LING1,2, HUAN WANG1,2, FEIMIAO WANG1,2, FANG XU1,2,*

1 School of Basic Medicine, Medical Genetics and Cell Biology, Ningxia Medical University, Yinchuan, 750004, China
2 Key Laboratory of Reproduction and Genetics, Ningxia Medical University, Yinchuan, 750004, China
3 Department of Human Anatomy and Histology Embryology, Ningxia Medical University, Yinchuan, 750004, China

* Corresponding Author: FANG XU. Email: email

(This article belongs to the Special Issue: Bioinformatics Study of Diseases)

BIOCELL 2023, 47(3), 607-618. https://doi.org/10.32604/biocell.2023.026445

Abstract

Background: Colon adenocarcinoma (COAD) is the second leading cause of cancer death worldwide thus, identification of COAD biomarkers is critical. Mitotic Arrest Deficient 2 Like 2 (MAD2L2) is a key factor in mammalian DNA damage repair and is highly expressed in many malignant tumors. This is a comprehensive study of MAD2L2 expression, its diagnostic value, prognostic analysis, potential biological function, and impact on the immune system of patients with COAD. Methods: Gene expression, clinical relevance, prognostic analysis, diagnostic value, GO/KEGG cluster analysis, data obtained from TCGA, and bioinformatics statistical analysis were performed using the R package. Immune responses to MAD2L2 expression in COAD were analyzed using TIMER. The expression of MAD2L2 in HCT116 cells induced by the inflammatory factor TNF-α was detected using Western blot. Results: Our results underscore the clinical diagnostic value and potential biological significance of MAD2L2 in patients with COAD. A high level of MAD2L2 expression has been found in COAD and correlated with tumor status and colon polyps. ROC curve analysis showed that MAD2L2 expression has high diagnostic value in COAD. Analysis of immune infiltration results showed that MAD2L2 expression was positively correlated with neutrophil levels. The western blot results demonstrated that MAD2L2 was dose-dependently present with TNF-α. GO/KEGG revealed that MAD2L2 overexpressed and coexpressed genes were mostly involved in biological functions, including hypoxia response, response to reduced oxygen levels, mitochondrial translation elongation, and other processes. Conclusion: MAD2L2 as a new COAD biomarker contributes to our understanding of how alterations in gene expression and the immunological environment contribute to the development of colon cancer. Following further investigation, MAD2L2 may prove to be a viable target factor for clinical diagnosis and therapy of COAD.

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APA Style
SUN, H., WANG, H., LI, X., HAO, Y., LING, J. et al. (2023). Increased mad2l2 expression predicts poor clinical outcome in colon adenocarcinoma. BIOCELL, 47(3), 607-618. https://doi.org/10.32604/biocell.2023.026445
Vancouver Style
SUN H, WANG H, LI X, HAO Y, LING J, WANG H, et al. Increased mad2l2 expression predicts poor clinical outcome in colon adenocarcinoma. BIOCELL . 2023;47(3):607-618 https://doi.org/10.32604/biocell.2023.026445
IEEE Style
H. SUN et al., “Increased MAD2L2 expression predicts poor clinical outcome in Colon Adenocarcinoma,” BIOCELL , vol. 47, no. 3, pp. 607-618, 2023. https://doi.org/10.32604/biocell.2023.026445



cc Copyright © 2023 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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