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Soy Protein Isolate Film by Incorporating Mandelic Acid as Well as Through Fermentation Mediated by Bacillus Subtilis

Rakesh Kumar1,*, Priya Rani1, K. Dinesh Kumar2

Department of Biotechnology, Central University of South Bihar, Gaya-824236, India.
Department of Materials Science & Engineering, Indian Institute of Technology, Patna 801106, India.

* Corresponding Author. Rakesh Kumar. Email: email; email .

Journal of Renewable Materials 2019, 7(2), 103-115. https://doi.org/10.32604/jrm.2019.00027

Abstract

Soy protein isolate (SPI) biopolymeric films were prepared by adding different contents of mandelic acid (1 to 5% wrt SPI) to glycerol plasticized SPI by solution casting method. Also, SPI was fermented by Bacillus subtilis to get fermented SPI films by solution casting. Molecular mass determination of mandelic acid incorporated and fermented SPI films was carried out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Mandelic acid incorporated and fermented SPI films were characterized by Fourier-transform infrared spectroscopy (FT-IR), dynamic mechanical analysis (DMA), tensile strength, water uptake and optical transmittance studies. Results indicated that incorporation of mandelic acid in SPI resulted in high tensile strength (8.03 MPa) and high α-relaxation (Tα) as well as low water uptake. On the other hand, films cannot be prepared from fermented SPI with SPI contents of 8% and 12%. However, film from fermented SPI with 16% SPI content could be prepared but it exhibited low tensile strength (3.18 MPa) and low Tα as well as high water uptake. The resulting mandelic acid incorporated SPI films were also subjected to antimicrobial studies. At all the concentration of mandelic acid, we can easily observe the antimicrobial effect in mandelic acid incorporated SPI films unlike fermented SPI films. This work will be helpful in fabricating antimicrobial SPI film from renewable resources.

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APA Style
Kumar, R., Rani, P., Kumar, K.D. (2019). Soy protein isolate film by incorporating mandelic acid as well as through fermentation mediated by bacillus subtilis. Journal of Renewable Materials, 7(2), 103-115. https://doi.org/10.32604/jrm.2019.00027
Vancouver Style
Kumar R, Rani P, Kumar KD. Soy protein isolate film by incorporating mandelic acid as well as through fermentation mediated by bacillus subtilis. J Renew Mater. 2019;7(2):103-115 https://doi.org/10.32604/jrm.2019.00027
IEEE Style
R. Kumar, P. Rani, and K.D. Kumar "Soy Protein Isolate Film by Incorporating Mandelic Acid as Well as Through Fermentation Mediated by Bacillus Subtilis," J. Renew. Mater., vol. 7, no. 2, pp. 103-115. 2019. https://doi.org/10.32604/jrm.2019.00027

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cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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