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Two Routes to Produce Chitosan from Agaricus bisporus

Abdelghani Hassainia1, Hamid Satha1,*, Boufi Sami2

1 LSPN, University of 8 Mai 1945, BP 401, Guelma, 24000, Algeria
2 Faculty of Science-LMSE, University of Sfax, Sfax, 3000, Tunisia

* Corresponding Author: Hamid Satha. Email: email

Journal of Renewable Materials 2020, 8(1), 101-111.


Two methods were used to produce chitosan by deacetylation of chitin which was extracted from Agaricus bisporus stipes. The first one gives chitosan 1 with low yield of 2.5%, degree of acetylation (DA) of 4%, molecular weight (MW) of 2.973 × 105 (g/mol). The second route produces chitosan 2 with higher yield of 41%, degree of acetylation (DA) of 17.23%, molecular weight (MW) of 2.939 × 105 (g/mol). Both chitosans were characterized by XRD, FTIR, 1 H-NMR spectroscopy nuclear magnetic resonance of proton. The molecular weight (MW) was determined by size exclusion chromatography (SEC). Thermal analysis shows that both chitosans have moisture content lower than 10%. However chitosan 2 has the less ash % which is the quality grade for chitosan medical applications. Accordingly, fungal chitosan 2 could have potential medical and agricultural applications.


Cite This Article

Hassainia, A., Satha, H., Sami, B. (2020). Two Routes to Produce Chitosan from Agaricus bisporus. Journal of Renewable Materials, 8(1), 101–111.


cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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