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Oct-1 Mediates ACTH-Induced Proliferation of Vascular Smooth Muscle Cells

Qian Xiao1, Xia Tang1, Yuanxiu Chen1, Han Bao1, Lizhi Gao1,2, Xiaobo Gong3,*, Ping Zhang1,4,*
Institute of Mechanobiology and Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Advanced Industrial Technology Research Institute, Shanghai Jiao Tong University, Shanghai, 200240, China.
Key Laboratory of Hydrodynamics (Ministry of Education), Department of Engineering Mechanics, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.
National Experimental Teaching Demonstration Center of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
* Corresponding Authors: Xiaobo Gong. Email: ; Ping Zhang. Email: .

Molecular & Cellular Biomechanics 2019, 16(3), 199-210. https://doi.org/10.32604/mcb.2019.07107

Abstract

Adrenocorticotrophic hormone (ACTH), a 39-amino acid peptide hormone, has been reported in the appreciation of the proliferation of vascular smooth muscle cells (VSMCs), however, the mechanism in molecular scale supporting the appreciation remains to be elucidated. In this study, we observed that the protein expression levels of ACTH at 24 h after exposure to 15% cyclic stretch were significantly higher than that after 5% cyclic stretch. When VSMCs were treated with 1000 nM ACTH directly, Oct-1 and lamin B1 expression were both up-regulated associating with each other, and the presence of Oct-1 was found shuttling between the cytosol and nucleus. When we silenced Oct-1 expression with RNA interference, the proliferation of VSMCs decreases significantly, which also validates a dominant contribution of Oct-1 in ACTH-induced VSMC proliferation. We further screened the target molecules of Oct-1 related to the proliferation with ingenuity pathway analysis (IPA), and found that superoxide dismutase 1 (SOD1) was significantly induced by ACTH stimulation yet suppressed by Oct-1 interference. All these findings in the present study highlight a new molecular mechanism that ACTH up-regulates Oct-1 expression and activates the protein expression of downstream target SOD1, finally induces the VSMC proliferation. The present work proved Octamer transcription factor-1 (Oct-1) as a key transcription factor in the mechanical regulation of VSMC proliferation, which in turn, provide a new target for the treatment of hypertension.

Keywords

Oct-1, proliferation, ACTH, VSMCs, cyclic strech, lamin B1

Cite This Article

Xiao, Q., Tang, X., Chen, Y., Bao, H., Gao, L. et al. (2019). Oct-1 Mediates ACTH-Induced Proliferation of Vascular Smooth Muscle Cells. Molecular & Cellular Biomechanics, 16(3), 199–210.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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