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HBx Downregulates miR-422a Expression via Activation of FOXG1/Q1/E1 in HepG2 Cells

Xiaofan Deng1,2, Yamei Yang2,3, Xianfeng Gan2,3, Gang Wu2,3,*

1 Organ Transplantation Center, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China
2 Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, 610072, China
3 Department of Hepatobiliary Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China

* Corresponding Author: Gang Wu. Email: email

Oncologie 2021, 23(2), 251-258. https://doi.org/10.32604/Oncologie.2021.016900

Abstract

microRNA-422a (miR-422a) is downregulated in many hematopoietic tumors and solid tumors including hepatocellular carcinoma. We previously demonstrated that hepatitis B virus X protein (HBx) downregulated expression of miR-422a in HCC cell line HepG2 in vitro. However, we explore the mechanisms underlying this action. Forkhead box proteins (FOX) G1/Q1/E1 are known to negatively regulate miR-422a expression, and this prompted us to determine whether HBx suppresses miR-422a expression via activation of FOXG1/Q1/E1. The relationship between FOXG1/Q1/E1 and miR-422a in HepG2 cells stably expressing HBx was assessed with qRT-PCR. The correlation between HBx and FOXG1/Q1/E1 was determined with qRT-PCR and western blot in vitro. The cell cycle and CCK-8 assays were used to elucidate the consequence of miR-422a transfection in HepG2-hbx cells. FOXG1/Q1/E1 activated by HBx was found to be responsible, at least in part, for the downregulation of miR-422a in HepG2 cells. miR-422a transfection hampered the growth of HepG2-hbx cells by arresting cells in G1 phase. Both FOXG1/Q1/E1 and miR-422a may be suitable molecular targets for treatment of HBV-infected HCC.

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APA Style
Deng, X., Yang, Y., Gan, X., Wu, G. (2021). Hbx downregulates mir-422a expression via activation of FOXG1/Q1/E1 in hepg2 cells. Oncologie, 23(2), 251-258. https://doi.org/10.32604/Oncologie.2021.016900
Vancouver Style
Deng X, Yang Y, Gan X, Wu G. Hbx downregulates mir-422a expression via activation of FOXG1/Q1/E1 in hepg2 cells. Oncologie . 2021;23(2):251-258 https://doi.org/10.32604/Oncologie.2021.016900
IEEE Style
X. Deng, Y. Yang, X. Gan, and G. Wu "HBx Downregulates miR-422a Expression via Activation of FOXG1/Q1/E1 in HepG2 Cells," Oncologie , vol. 23, no. 2, pp. 251-258. 2021. https://doi.org/10.32604/Oncologie.2021.016900



cc Copyright © 2021 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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