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miRNA-497 Negatively Regulates the Growth and Motility of Chondrosarcoma Cells by Targeting Cdc25A

Yandong Lu*1, Fangguo Li*1, Tao Xu, Jie Sun*1

* Department of Orthopaedic Traumatology, Tianjin Hospital, Tianjin, China
† Department of Orthopaedics, Jixian People’s Hospital, Tianjin, China

Oncology Research 2015, 23(4), 155-163. https://doi.org/10.3727/096504016X14519157902681

A retraction of this article was approved in:

Retraction: miRNA-497 negatively regulates the growth and motility of chondrosarcoma cells by targeting Cdc25A
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Abstract

Chondrosarcoma (CHS) is the second most common malignant bone sarcoma with increased risk of invasion and metastasis. However, the regulatory mechanisms of CHS tumorigenesis remain unknown. Here we investigated the novel role of miR-497 in regulating chondrosarcoma cell growth and cell cycle arrest. RT-PCR analysis showed that the expression of miR-497 is aberrantly downregulated in human chondrosarcoma samples and cells. After transfection with miR-497 mimic or antagomir, the proliferation and apoptosis of JJ012 and OUMS-27 chondrosarcoma cells were determined by CCK-8 assay and flow cytometric analysis, respectively. Results showed that the proliferation capacity of JJ012 and OUMS-27 cells was significantly decreased by miR-497 overexpression but increased by miR-497 repression. Apoptosis in both cell types was remarkably enhanced by miR-497 mimic but inhibited by miR-497 antagomir. By bioinformatics and luciferase reporter analysis, Cdc25A was proven to be a direct target of miR-497 in chondrosarcoma cells. Further studies indicated that miR-497 modulates the growth of chondrosarcoma cells by targeting Cdc25A, in which the cell cycle inhibitor p21 is involved through a p53-independent pathway. In conclusion, we demonstrated that miR-497 represents a potential tumor suppressor in human chondrosarcoma that regulates the growth of chondrosarcoma cells by targeting Cdc25A. This may provide a novel therapeutic target for chondrosarcoma.

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APA Style
Lu, Y., Li, F., Xu, T., Sun, J. (2015). Mirna-497 negatively regulates the growth and motility of chondrosarcoma cells by targeting cdc25a. Oncology Research, 23(4), 155-163. https://doi.org/10.3727/096504016X14519157902681
Vancouver Style
Lu Y, Li F, Xu T, Sun J. Mirna-497 negatively regulates the growth and motility of chondrosarcoma cells by targeting cdc25a. Oncol Res. 2015;23(4):155-163 https://doi.org/10.3727/096504016X14519157902681
IEEE Style
Y. Lu, F. Li, T. Xu, and J. Sun, “miRNA-497 Negatively Regulates the Growth and Motility of Chondrosarcoma Cells by Targeting Cdc25A,” Oncol. Res., vol. 23, no. 4, pp. 155-163, 2015. https://doi.org/10.3727/096504016X14519157902681



cc Copyright © 2015 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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