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Long Noncoding RNA LINC0086 Functions as a Tumor Suppressor in Nasopharyngeal Carcinoma by Targeting miR-214

Jie Guo*†, Jinqi Ma, Guosheng Zhao, Guocai Li, Yunfeng Fu, Yanwei Luo, Rong Gui

* Department of Preventive Oncology, State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, P.R. China
† Department of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-Sen University, Guangzhou, P.R. China
‡ Department of Blood Transfusion, The Third Xiangya Hospital of Central South University, Changsha, P.R. China

Oncology Research 2017, 25(7), 1189-1197. https://doi.org/10.3727/096504017X14865126670075

Abstract

Nasopharyngeal carcinoma (NPC) is a distinct head and neck cancer, which is occurring at a high frequency in Southern China. Emerging studies have shown that long noncoding RNAs (lncRNAs) play a critical role in carcinogenesis and progression. In this study, we established a comprehensive lncRNA profile in NPC and found that 35 lncRNAs were differentially expressed in NPC. We found that LINC0086 was decreased in NPC patient serum samples and tissues. The Kaplan–Meier survival curve showed that patients with high LINC0086 expression had a higher survival rate than those with low LINC0086 expression. LINC0086 expression was associated with NPC histological grade, lymph node metastasis, and clinical stage. Upregulation of LINC0086 inhibited cancer cell proliferation and promoted apoptosis. In addition, upregulation of LINC0086 dramatically decreased the expression of miR-214, an oncogene in several cancers, in C666-1 and HK-1 cells. An miR-214 binding site was found in the 3'-UTR of LINC0086. We also validated that both miR-214 and LINC0086 presented in the RISC complex, demonstrating that LINC0086 could decrease miR-214 expression by directly interacting with miR-214. Furthermore, the suppressive effects of LINC0086 on NPC cell growth were reversed by overexpression of miR-214 in vitro and in vivo. Thus, our study reports a novel mechanism underlying NPC carcinogenesis and provides a potential novel diagnosis and treatment biomarker for NPC.

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APA Style
Guo, J., Ma, J., Zhao, G., Li, G., Fu, Y. et al. (2017). Long noncoding RNA LINC0086 functions as a tumor suppressor in nasopharyngeal carcinoma by targeting mir-214. Oncology Research, 25(7), 1189-1197. https://doi.org/10.3727/096504017X14865126670075
Vancouver Style
Guo J, Ma J, Zhao G, Li G, Fu Y, Luo Y, et al. Long noncoding RNA LINC0086 functions as a tumor suppressor in nasopharyngeal carcinoma by targeting mir-214. Oncol Res. 2017;25(7):1189-1197 https://doi.org/10.3727/096504017X14865126670075
IEEE Style
J. Guo et al., “Long Noncoding RNA LINC0086 Functions as a Tumor Suppressor in Nasopharyngeal Carcinoma by Targeting miR-214,” Oncol. Res., vol. 25, no. 7, pp. 1189-1197, 2017. https://doi.org/10.3727/096504017X14865126670075



cc Copyright © 2017 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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