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ARTICLE
Highly Expressed Antisense Noncoding RNA in the INK4 Locus Promotes Growth and Invasion of Renal Clear Carcinoma Cells via the β-Catenin Pathway
Qingchun Li*, Yuan Tian†, Guangrui Hu†, Yun Liang†, Wei Bai†, Hongjun Li†
* Department of Gastrointestinal Colorectal and Anal Surgery, China–Japan Union Hospital of Jilin University,
Changchun, P.R. China
† Center of Physical Examination, China–Japan Union Hospital of Jilin University, Changchun, P.R. China
Oncology Research 2017, 25(8), 1373-1382. https://doi.org/10.3727/096504017X14878509668646
Abstract
Long noncoding RNA (lncRNA) antisense noncoding RNA in the INK4 locus (ANRIL) is involved in several
human cancers. However, the role of ANRIL in renal cell carcinoma (RCC) remains unclear. This study aimed
to explore whether, and how, ANRIL affects the progression of RCC. First, the expression of ANRIL in clinical
tumor tissues and four kinds of RCC cell lines was evaluated. After transfection, cell viability, colony number,
apoptosis, migration, and invasion were assessed. The expression of proteins related to apoptosis, epithelial-tomesenchymal transition (EMT), and the β-catenin signaling pathway was then assessed. In addition, the effect
of IWR-endo (β-catenin inhibitor) on cell viability, migration, and invasion, as well as β-catenin expression,
was also evaluated. The results showed that ANRIL was highly expressed in RCC tissues and RCC cell lines.
ANRIL significantly promoted cell proliferation, migration, invasion, and EMT but inhibited cell apoptosis.
Additionally, the expression levels of β-catenin, Ki-67, glycogen synthase kinase 3β (GSK-3β), phosphorylated GSK-3β, T-cell transcription factor 4 (TCF-4), and leukemia enhancer factor 1 (LEF-1) were all markedly
upregulated by ANRIL. The effect of ARNIL silencing was opposite to that of ANRIL overexpression. The
effect of ARNIL on proliferation, migration, and invasion of RCC cells was found to be reversed by IWRendo. In conclusion, ANRIL, which is highly expressed in RCC, acted as a carcinogen in RCC cells through
the activation of the β-catenin pathway.
Keywords
Cite This Article
APA Style
Li, Q., Tian, Y., Hu, G., Liang, Y., Bai, W. et al. (2017). Highly expressed antisense noncoding RNA in the INK4 locus promotes growth and invasion of renal clear carcinoma cells via the β-catenin pathway. Oncology Research, 25(8), 1373-1382. https://doi.org/10.3727/096504017X14878509668646
Vancouver Style
Li Q, Tian Y, Hu G, Liang Y, Bai W, Li H. Highly expressed antisense noncoding RNA in the INK4 locus promotes growth and invasion of renal clear carcinoma cells via the β-catenin pathway. Oncol Res. 2017;25(8):1373-1382 https://doi.org/10.3727/096504017X14878509668646
IEEE Style
Q. Li, Y. Tian, G. Hu, Y. Liang, W. Bai, and H. Li "Highly Expressed Antisense Noncoding RNA in the INK4 Locus Promotes Growth and Invasion of Renal Clear Carcinoma Cells via the β-Catenin Pathway," Oncol. Res., vol. 25, no. 8, pp. 1373-1382. 2017. https://doi.org/10.3727/096504017X14878509668646