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Regulation of Acetate Utilization by Monocarboxylate Transporter 1 (MCT1) in Hepatocellular Carcinoma (HCC)

Jeong Yong Jeon*1, Misu Lee*†1, Sang Hyun Whang*, Jung-Whan Kim, Arthur Cho*, Mijin Yun*

* Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea
† Division of Life Science, College of Life Science and Bioengineering, Incheon National University, Incheon, South Korea
‡ Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX, USA
1 These authors provided equal contribution to this work.

Oncology Research 2018, 26(1), 71-81. https://doi.org/10.3727/096504017X14902648894463

Abstract

Altered energy metabolism is a biochemical fingerprint of cancer cells. Hepatocellular carcinoma (HCC) shows reciprocal [18F]fluorodeoxyglucose (FDG) and [11C]acetate uptake, as revealed by positron emission tomography/computed tomography (PET/CT). Previous studies have focused on the role of FDG uptake in cancer cells. In this study, we evaluated the mechanism and roles of [11C]acetate uptake in human HCCs and cell lines. The expression of monocarboxylate transporters (MCTs) was assessed to determine the transporters of [11C]acetate uptake in HCC cell lines and human HCCs with different [11C]acetate uptake. Using two representative cell lines with widely different [11C]acetate uptake (HepG2 for high uptake and Hep3B for low uptake), changes in [11C]acetate uptake were measured after treatment with an MCT1 inhibitor or MCT1- targeted siRNA. To verify the roles of MCT1 in cells, oxygen consumption rate and the amount of lipid synthesis were measured. HepG2 cells with high [11C]acetate uptake showed higher MCT1 expression than other HCC cell lines with low [11C]acetate uptake. MCT1 expression was elevated in human HCCs with high [11C] acetate uptake compared to those with low [11C]acetate uptake. After blocking MCT1 with AR-C155858 or MCT1 knockdown, [11C]acetate uptake in HepG2 cells was significantly reduced. Additionally, inhibition of MCT1 suppressed mitochondrial oxidative phosphorylation, lipid synthesis, and cellular proliferation in HCC cells with high [11C]acetate uptake. MCT1 may be a new therapeutic target for acetate-dependent HCCs with high [11C]acetate uptake, which can be selected by [11C]acetate PET/CT imaging in clinical practice.

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APA Style
Jeon, J.Y., Lee, M., Whang, S.H., Kim, J., Cho, A. et al. (2018). Regulation of acetate utilization by monocarboxylate transporter 1 (MCT1) in hepatocellular carcinoma (HCC). Oncology Research, 26(1), 71-81. https://doi.org/10.3727/096504017X14902648894463
Vancouver Style
Jeon JY, Lee M, Whang SH, Kim J, Cho A, Yun M. Regulation of acetate utilization by monocarboxylate transporter 1 (MCT1) in hepatocellular carcinoma (HCC). Oncol Res. 2018;26(1):71-81 https://doi.org/10.3727/096504017X14902648894463
IEEE Style
J.Y. Jeon, M. Lee, S.H. Whang, J. Kim, A. Cho, and M. Yun "Regulation of Acetate Utilization by Monocarboxylate Transporter 1 (MCT1) in Hepatocellular Carcinoma (HCC)," Oncol. Res., vol. 26, no. 1, pp. 71-81. 2018. https://doi.org/10.3727/096504017X14902648894463



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