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MicroRNA-103 Promotes Proliferation and Inhibits Apoptosis in Spinal Osteosarcoma Cells by Targeting p57

Xuesong Wang*, Yong Lin, Lei Peng, Ruifu Sun*, Xiaojin Gong*, Jinlong Du*, Xiugong Zhang*

* Department of Spinal, Qingdao Central Hospital, Qingdao, P.R. China
† Department of Spinal, Qingdao Municipal Hospital, Qingdao, P.R. China
‡ Library of Qingdao Central Hospital, Qingdao, P.R. China

Oncology Research 2018, 26(6), 933-940. https://doi.org/10.3727/096504017X15144741233346

Abstract

Osteosarcoma is one of the most aggressive malignancies with poor prognosis rates. Many studies have demonstrated that miRNAs were involved in osteosarcoma, but the role of miR-103a in osteosarcoma remains elusive. In this study, we detected the expression levels of miR-103 in osteosarcoma and non-osteosarcoma tissues and cell lines. The binding effect of miR-103 on p57 was detected by luciferase reporter assay. After altering expressions of miR-103 or p57, viability, migration, invasion, and apoptosis of MG63 cells and expressions of proteins related with the JNK/STAT and mTOR pathways were all detected. We found the higher expression of miR-103 in osteosarcoma tissues and cell lines compared with non-osteosarcoma tissues and cell lines. miR-103 overexpression promoted survival, migration, and invasion of MG63 cells. Knockdown of miR-103a inhibited cell survival, migration, and invasion by upregulating the expression of p57, which was a target of miR-103. Moreover, miR-103a overexpression activated the JNK/STAT and mTOR pathways probably through inhibiting p57 expression. In conclusion, miR-103a acted as an oncogene in osteosarcoma, probably through activating the JNK/STAT and mTOR pathways by inhibiting p57 expression.

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APA Style
Wang, X., Lin, Y., Peng, L., Sun, R., Gong, X. et al. (2018). Microrna-103 promotes proliferation and inhibits apoptosis in spinal osteosarcoma cells by targeting p57. Oncology Research, 26(6), 933-940. https://doi.org/10.3727/096504017X15144741233346
Vancouver Style
Wang X, Lin Y, Peng L, Sun R, Gong X, Du J, et al. Microrna-103 promotes proliferation and inhibits apoptosis in spinal osteosarcoma cells by targeting p57. Oncol Res. 2018;26(6):933-940 https://doi.org/10.3727/096504017X15144741233346
IEEE Style
X. Wang et al., "MicroRNA-103 Promotes Proliferation and Inhibits Apoptosis in Spinal Osteosarcoma Cells by Targeting p57," Oncol. Res., vol. 26, no. 6, pp. 933-940. 2018. https://doi.org/10.3727/096504017X15144741233346



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