Open Access
ARTICLE
The Interaction Between lncRNA SNHG1 and miR-140 in Regulating Growth and Tumorigenesis via the TLR4/NF-kB Pathway in Cholangiocarcinoma
Zhen Li*1,
Xin Li*1,
Xiao Du†, Henghui Zhang†, Zhengyang Wu*, Kewei Ren*, Xinwei Han*
* Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, P.R. China
† Beijing Genecast Biotechnology Co., Beijing, P.R. China
Oncology Research 2019, 27(6), 663-672. https://doi.org/10.3727/096504018X15420741307616
Abstract
Cholangiocarcinoma (CCA) is the second most common primary hepatobiliary carcinoma. The long noncoding
RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) has been reported to contribute to the progression
of multiple cancers. Nonetheless, the functions and hidden mechanism of SNHG1 remain unclear in CCA. In
this study, the SNHG1 levels were boosted in CCA cell lines, and knockdown of SNHG1 repressed CCA cell
proliferation and invasion in vitro. The data also demonstrated that miR-140 could act as a target of SNHG1 in
CCA and inhibited CCA cell proliferation and invasion, whereas the inhibition effects were relieved by overexpression of SNHG1. In addition, Toll-like receptor 4 (TLR4), an NF- B-activating signal, was identified
to be a target of miR-140. SNHG1, as a competing endogenous RNA (ceRNA) for miR-140, enhanced TLR4
expression and activated NF- B signaling, thereby regulating growth and tumorigenesis in CCA. Animal
experiments further confirmed this conclusion. Collectively, these findings not only uncovered a key role of
SNHG1/miR-140/TLR4/NF- B signaling axis in CCA tumorigenesis and progression but also denoted the
probable utilization of SNHG1 as a therapeutic target for CCA.
Keywords
Cite This Article
Li, Z., Li,
. X., Du,
. X., Zhang, H., Wu, Z. et al. (2019). The Interaction Between lncRNA SNHG1 and miR-140 in Regulating Growth and Tumorigenesis via the TLR4/NF-kB Pathway in Cholangiocarcinoma.
Oncology Research, 27(6), 663–672. https://doi.org/10.3727/096504018X15420741307616