Open Access
ARTICLE
MafF Is Regulated via the circ-ITCH/miR-224-5p Axis and Acts as a Tumor Suppressor in Hepatocellular Carcinoma
Minhua Wu*1, Xubin Deng†1, Yu Zhong‡1, Li Hu*, Xiujuan Zhang§, Yanqin Liang*, Xiaofang Li¶, Xiaoxia Ye*
* Department of Histology and Embryology, Guangdong Medical University, Zhanjiang, P.R. China
† Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, P.R. China
‡ Analysis Center, Guangdong Medical University, Zhanjiang, P.R. China
§ Department of Physiology, Guangdong Medical University, Zhanjiang, P.R. China
¶ Pathological Diagnosis and Research Center, Affiliated Hospital of Guangdong Medical University,
Zhanjiang, P.R. China
Oncology Research 2020, 28(3), 299-309. https://doi.org/10.3727/096504020X15796890809840
Abstract
MafF is a member of the basic leucine zipper (bZIP) transcription factor Maf family and is commonly
downregulated in multiple cancers. But the expression and function of MafF in hepatocellular carcinoma
(HCC) remain unclear. In this study, we investigated the relationship between endogenous MafF expression
and HCC progression and explored the regulatory mechanism of MafF expression in HCC. We found that MafF
decreased in HCC tissues and cells. Lentivirus-mediated MafF overexpression inhibited HCC cell proliferation
and induced cell apoptosis. Bioinformatics analysis and luciferase assay identified MafF as a direct target of
miR-224-5p. RNA pull-down assay demonstrated that circular RNA circ-ITCH could sponge miR-224-5p specifically in HCC. The rescue experiments further elucidated that the expression and antitumor effects of MafF
could be regulated via the circ-ITCH/miR-224-5p axis. This study verified that MafF acted as a tumor suppressor in HCC and revealed the upstream regulation mechanism of MafF, which provided a new perspective for
potential therapeutic targets of HCC.
Keywords
Cite This Article
Wu, M., Deng, X., Zhong, Y., Hu, L., Zhang, X. et al. (2020). MafF Is Regulated via the circ-ITCH/miR-224-5p Axis and Acts as a Tumor Suppressor in Hepatocellular Carcinoma.
Oncology Research, 28(3), 299–309.