Open Access
ARTICLE
MicroRNA-188-5p targeting Forkhead Box L1 promotes colorectal cancer progression via activating Wnt/β-catenin signaling
JIALIN WU1,2,#, ZEHONG CHEN3, WENWEI LIU1,#, YONGXIN ZHANG1, WEI FENG1, YUJIE YUAN1, JINNING YE1, LIANG WANG1, SHIRONG CAI1, YULONG HE1, SUIJING WU4,*, WU SONG1,*
1 Center of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
2 Department of Hepatobiliary and Pancreatic Surgery, Union Shenzhen Hospital, Huazhong University of Science and Technology, Shenzhen, China
3 Department of Gastrointestinal Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
4 Department of Hematology, Guangdong General Hospital, Guangzhou, China
* Corresponding Authors: Suijing Wu, ; Wu Song,
# Jialin Wu, and Wenwei Liu contributed equally to this work
Oncology Research 2021, 29(2), 119-128. https://doi.org/10.32604/or.2022.03178
Received 19 December 2019; Accepted 27 August 2020; Issue published 13 July 2022
Abstract
Objective: MicroRNA-188-5p (miR-188) enhances oncologic progression in various human malignancies. This
study aimed to explore its role in colorectal cancer (CRC).
Materials and Methods: Human CRC tissues paired with
normal tissues, and several CRC cell lines were utilized. Real-time quantitative PCR was applied to measure the
expression of miR-188. Overexpression and knockdown were used to access the function of miR-188 and to
investigate whether FOXL1/Wnt signaling mediates such function. The proliferation, migration and invasion of cancer
cells were evaluated by CCK8, wound-healing and transwell assays, respectively. Whether FOXL1 acted as a direct
target of miR-188 was verified by dual-luciferase reporter assays.
Results: Levels of miR-188 were upregulated in CRC
tissues than in paired-normal tissues, as well as in various CRC cell lines. High expression of miR-188 was strongly
associated with advanced tumor stage, accompanied with significant tumor cell proliferation, invasion and migration.
It was confirmed that FOXL1 played positive crosstalk between miR-188 regulation and downstream Wnt/β-catenin
signaling activation.
Conclusions: All findings indicate that miR-188 promotes CRC cell proliferation and invasion
through targeting FOXL1/Wnt signaling and could be served as a potential therapeutic target for human CRC in the
future.
Keywords
Cite This Article
WU, J., CHEN, Z., LIU, W., ZHANG, Y., FENG, W. et al. (2021). MicroRNA-188-5p targeting Forkhead Box L1 promotes colorectal cancer progression via activating Wnt/β-catenin signaling.
Oncology Research, 29(2), 119–128. https://doi.org/10.32604/or.2022.03178