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MicroRNA-188-5p targeting Forkhead Box L1 promotes colorectal cancer progression via activating Wnt/β-catenin signaling

JIALIN WU1,2,#, ZEHONG CHEN3, WENWEI LIU1,#, YONGXIN ZHANG1, WEI FENG1, YUJIE YUAN1, JINNING YE1, LIANG WANG1, SHIRONG CAI1, YULONG HE1, SUIJING WU4,*, WU SONG1,*

1 Center of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
2 Department of Hepatobiliary and Pancreatic Surgery, Union Shenzhen Hospital, Huazhong University of Science and Technology, Shenzhen, China
3 Department of Gastrointestinal Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China
4 Department of Hematology, Guangdong General Hospital, Guangzhou, China

* Corresponding Authors: Suijing Wu, email; Wu Song, email
# Jialin Wu, and Wenwei Liu contributed equally to this work

Oncology Research 2021, 29(2), 119-128. https://doi.org/10.32604/or.2022.03178

Abstract

Objective: MicroRNA-188-5p (miR-188) enhances oncologic progression in various human malignancies. This study aimed to explore its role in colorectal cancer (CRC). Materials and Methods: Human CRC tissues paired with normal tissues, and several CRC cell lines were utilized. Real-time quantitative PCR was applied to measure the expression of miR-188. Overexpression and knockdown were used to access the function of miR-188 and to investigate whether FOXL1/Wnt signaling mediates such function. The proliferation, migration and invasion of cancer cells were evaluated by CCK8, wound-healing and transwell assays, respectively. Whether FOXL1 acted as a direct target of miR-188 was verified by dual-luciferase reporter assays. Results: Levels of miR-188 were upregulated in CRC tissues than in paired-normal tissues, as well as in various CRC cell lines. High expression of miR-188 was strongly associated with advanced tumor stage, accompanied with significant tumor cell proliferation, invasion and migration. It was confirmed that FOXL1 played positive crosstalk between miR-188 regulation and downstream Wnt/β-catenin signaling activation. Conclusions: All findings indicate that miR-188 promotes CRC cell proliferation and invasion through targeting FOXL1/Wnt signaling and could be served as a potential therapeutic target for human CRC in the future.

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APA Style
WU, J., CHEN, Z., LIU, W., ZHANG, Y., FENG, W. et al. (2021). Microrna-188-5p targeting forkhead box L1 promotes colorectal cancer progression via activating wnt/β-catenin signaling. Oncology Research, 29(2), 119-128. https://doi.org/10.32604/or.2022.03178
Vancouver Style
WU J, CHEN Z, LIU W, ZHANG Y, FENG W, YUAN Y, et al. Microrna-188-5p targeting forkhead box L1 promotes colorectal cancer progression via activating wnt/β-catenin signaling. Oncol Res. 2021;29(2):119-128 https://doi.org/10.32604/or.2022.03178
IEEE Style
J. WU et al., "MicroRNA-188-5p targeting Forkhead Box L1 promotes colorectal cancer progression via activating Wnt/β-catenin signaling," Oncol. Res., vol. 29, no. 2, pp. 119-128. 2021. https://doi.org/10.32604/or.2022.03178



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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