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Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microRNA-548a-3p/CDK4 axis as a competitive endogenous RNA

YANG ZHANG, LIXIA MA, TINGTING ZHANG, PEIDONG LI, JIABIN XU, ZHUO WANG*

Department of Oncology, Jilin Province Cancer Hospital, Jilin, 130012, China

* Corresponding Author: Zhuo Wang, email

Oncology Research 2021, 29(2), 129-139. https://doi.org/10.32604/or.2022.03563

Abstract

In this study, we mainly focus on probing expression profile and detailed functions of long non-coding RNA TFAP2A antisense RNA 1 (TFAP2A-AS1) in non-small cell lung cancer (NSCLC). Moreover, the mechanisms played by TFAP2A-AS1 were unraveled comprehensively. Herein, a notable overexpressed TFAP2A-AS1 in NSCLC was observed by TCGA and our own cohort. An increased TFAP2A-AS1 level displayed a negative correlation with the overall survival of patients with NSCLC. Loss-of-function approaches illustrated that the absence of TFAP2A-AS1 weakened NSCLC cell proliferation, colony formation, migration and invasion in vitro. Also, interference of TFAP2A-AS1 caused in vivo tumor growth suppression. Mechanistically, TFAP2A-AS1 could negative regulate microRNA-584-3p (miR-584-3p) as a competitive endogenous RNA. Furthermore, cyclin-dependent kinase 4 (CDK4), a direct target of miR-584-3p, was positively controlled by TFAP2A-AS1 in a miR-5184-3p-dependent manner. Rescue function experiments corroborated that the anticancer activities of TFAP2A-AS1 deficient on the oncogenicity of NSCLC cells were reversed by downregulating miR-584-3p or overexpressing CDK4. To sum up, TFAP2A-AS1 exhibits cancerpromoting roles in NSCLC through the adjustment of miR-584-3p/CDK4 axis.

Keywords

TFAP2A antisense RNA 1, lung cancer, ceRNA pathway, therapeutic target

Cite This Article

APA Style
ZHANG, Y., MA, L., ZHANG, T., LI, P., XU, J. et al. (2021). Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microRNA-548a-3p/CDK4 axis as a competitive endogenous RNA. Oncology Research, 29(2), 129–139. https://doi.org/10.32604/or.2022.03563
Vancouver Style
ZHANG Y, MA L, ZHANG T, LI P, XU J, WANG Z. Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microRNA-548a-3p/CDK4 axis as a competitive endogenous RNA. Oncol Res. 2021;29(2):129–139. https://doi.org/10.32604/or.2022.03563
IEEE Style
Y. ZHANG, L. MA, T. ZHANG, P. LI, J. XU, and Z. WANG, “Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microRNA-548a-3p/CDK4 axis as a competitive endogenous RNA,” Oncol. Res., vol. 29, no. 2, pp. 129–139, 2021. https://doi.org/10.32604/or.2022.03563



cc Copyright © 2021 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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