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Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microRNA-548a-3p/CDK4 axis as a competitive endogenous RNA

YANG ZHANG, LIXIA MA, TINGTING ZHANG, PEIDONG LI, JIABIN XU, ZHUO WANG*

Department of Oncology, Jilin Province Cancer Hospital, Jilin, 130012, China

* Corresponding Author: Zhuo Wang, email

Oncology Research 2021, 29(2), 129-139. https://doi.org/10.32604/or.2022.03563

Abstract

In this study, we mainly focus on probing expression profile and detailed functions of long non-coding RNA TFAP2A antisense RNA 1 (TFAP2A-AS1) in non-small cell lung cancer (NSCLC). Moreover, the mechanisms played by TFAP2A-AS1 were unraveled comprehensively. Herein, a notable overexpressed TFAP2A-AS1 in NSCLC was observed by TCGA and our own cohort. An increased TFAP2A-AS1 level displayed a negative correlation with the overall survival of patients with NSCLC. Loss-of-function approaches illustrated that the absence of TFAP2A-AS1 weakened NSCLC cell proliferation, colony formation, migration and invasion in vitro. Also, interference of TFAP2A-AS1 caused in vivo tumor growth suppression. Mechanistically, TFAP2A-AS1 could negative regulate microRNA-584-3p (miR-584-3p) as a competitive endogenous RNA. Furthermore, cyclin-dependent kinase 4 (CDK4), a direct target of miR-584-3p, was positively controlled by TFAP2A-AS1 in a miR-5184-3p-dependent manner. Rescue function experiments corroborated that the anticancer activities of TFAP2A-AS1 deficient on the oncogenicity of NSCLC cells were reversed by downregulating miR-584-3p or overexpressing CDK4. To sum up, TFAP2A-AS1 exhibits cancerpromoting roles in NSCLC through the adjustment of miR-584-3p/CDK4 axis.

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APA Style
ZHANG, Y., MA, L., ZHANG, T., LI, P., XU, J. et al. (2021). Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microrna-548a-3p/cdk4 axis as a competitive endogenous RNA. Oncology Research, 29(2), 129-139. https://doi.org/10.32604/or.2022.03563
Vancouver Style
ZHANG Y, MA L, ZHANG T, LI P, XU J, WANG Z. Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microrna-548a-3p/cdk4 axis as a competitive endogenous RNA. Oncol Res. 2021;29(2):129-139 https://doi.org/10.32604/or.2022.03563
IEEE Style
Y. ZHANG, L. MA, T. ZHANG, P. LI, J. XU, and Z. WANG "Long noncoding RNA TFAP2A-AS1 exerts promotive effects in non-small cell lung cancer progression via controlling the microRNA-548a-3p/CDK4 axis as a competitive endogenous RNA," Oncol. Res., vol. 29, no. 2, pp. 129-139. 2021. https://doi.org/10.32604/or.2022.03563



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