Open Access

ARTICLE

Plant micropropagation in experimental lines of forage maize coming from crossing Zea mays L. and Zea diploperennis Iltis, Doebley and Guzmán

Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

Facultad de Agronomía UNLPam. Dirección: CC 300. (6300) Santa Rosa, La Pampa, Argentina.

* Corresponding Author:Address Correspondence to: María del Carmen Torroba, e-mail: ; Telefax: 02954-433092.

Phyton-International Journal of Experimental Botany 2008, 77(all), 93-102. https://doi.org/10.32604/phyton.2008.77.093

Abstract

An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the highest rate of regeneration in line L.850 was reached in the second subculture (1.8 plants) with subculture every 30 days, and in the fifth subculture (1.8 plants) with subculture every 15 days. In line L.769, the highest frequency of regeneration was reached in the fourth subculture (1.7 plants). Regeneration rates were low compared with somatic embryogenesis, but no somaclonal variations were detected in field plant phenotypes; a marked uniformity was detected within lines.

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