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Anatomical and chemical characteristics of the seed coat of Medicago sativa L. (alfalfa) cv. Baralfa 85 seeds and their association with seed dormancy

Galussi AA1, JA Argüello2, MM Cerana2, M Maximino3, ME Moya1
Facultad de Ciencias Agropecuarias, Universidad Nacional de Entre Ríos, Argentina.
Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Argentina.
Facultad Ingeniería Química, Universidad Nacional del Litoral, Argentina.
* Corresponding Authors:Address Correspondence to: Alberto A. Galussi, Facultad de Ciencias Agropecuarias UNER, Oro Verde, Ruta Prov. Nº11, km 10,5 (3101) Paraná, Entre Ríos, Argentina, e-mail: ;

Phyton-International Journal of Experimental Botany 2015, 84(1), 163-175. https://doi.org/10.32604/phyton.2015.84.163

Abstract

Seeds of alfalfa (Medicago sativa L.) can exhibit seedcoat imposed dormancy, which produces hard seeds within a seed lot. These seeds do not germinate because they do not imbibe water due to a barrier to water entry in the seed coat. The aim of this work was to analyze the anatomical and chemical characteristics of the testa of alfalfa seeds with respect to water permeability levels. The anatomy of seeds of the cv. Baralfa 85 was studied and structural substances, polyphenols, tannins and cutin present in the testa of seeds of different water permeability levels were determined. The anatomical characteristics of the seed coat and the proportions of components were found to determine the permeability level of the seed coat, an aspect that is associated with the physical seed dormancy level. Anatomically, increased thickness of the testa was associated with a lower permeability level. The difference may be attributed to the variation in cuticle thickness, length of macrosclereids and thickness of the cell wall, and presence and development of osteosclereids. From the physiological and chemical points of view, the mechanism of physical dormancy of the testa is explained by a greater amount of components that repel water and cement the cell wall, such as polyphenols, lignins, condensed tannins, pectic substances, and a lower proportion of cellulose and hemicellulose.

Keywords

Hard seeds, Permeability, Testa, Macrosclereids, Lignin.

Cite This Article

AA, G., Argüello, J., Cerana, M., Maximino, M., Moya, M. (2015). Anatomical and chemical characteristics of the seed coat of Medicago sativa L. (alfalfa) cv. Baralfa 85 seeds and their association with seed dormancy. Phyton-International Journal of Experimental Botany, 84(1), 163–175.

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This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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