Open Access
ARTICLE
Somatic embryogenesis induction from immature embryos of Sorghum bicolor L. (Moench)
Espinoza-Sánchez EA1, YA Sánchez-Peña2, JA Torres-Castillo3, EA García-Zambrano2, JE Treviño Ramírez2, F Zavala-García2, SR Sinagawa-García2
1 Laboratorio de Biotecnología I, Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Circuito Universitario S/N, Campus Universitario II, Chihuahua, Chihuahua,
México, C.P. 31125.
2 Universidad Autónoma de Nuevo León, Facultad de Agronomia, Laboratorio de Biotecnología, Francisco Villa S/N Col. Ex hacienda El Canadá, General Escobedo, Nuevo
León, México. C.P. 66050.
3 Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, División del Golfo No. 356. Colonia Libertad. Cd. Victoria, Tamaulipas, México, CP. 87019, México.
Address correspondence to: Sugey Ramona Sinagawa-García. Tel. 52-81-1340-4399 ext. 3517, Fax: 52-81-8397-4588 e-mail:
Phyton-International Journal of Experimental Botany 2018, 87(all), 105-112. https://doi.org/10.32604/phyton.2018.87.105
Abstract
In this study, we established an in vitro regeneration
system from immature embryos of four varieties of Sweet Sorghum
with biotechnological potential. A total of 17 treatments were assesses
in the induction of somatic embryogenic calli. We analyzed
the effect of Murashige y Skoog media supplemented with different
concentrations of plant growth regulators such as auxins and cytokinins.
The callus induction response was evaluated using three immature
embryos development stages and based on the number of
embryos with callus induction. Different responses were observed
in the embryos depending of the embryo age, culture condition,
media composition and variety. The results showed that embryos of
the middle stage had the best response in terms of calli induction.
Interestingly, more embryos responded to calli induction in light
presence. Complex media showed better response that minimal media
to callus induction, media complemented with 4 to 6 mg/L of
2,4-D combined with 1 mg/L of BAP were more efficient to callus
induction. With respect to variety, the best response to callus induction
was obtained with Atlas variety, followed by Keller and Kansas,
whereas Fortuna variety had lower response. In this work also, it was
obtained an efficient culture medium to shoots development because
until 90% of calli response to shoots regeneration. This study reports
relevant information to tissue culture protocols that can be used in
cereals in vitro cultivation as tool for biotechnological applications.
Keywords
Cite This Article
EA, E., Sánchez-Peña, Y., Torres-Castillo, J., García-Zambrano, E., Ramírez, J. T. et al. (2018). Somatic embryogenesis induction from immature embryos of
Sorghum bicolor L. (Moench).
Phyton-International Journal of Experimental Botany, 87(all), 105–112. https://doi.org/10.32604/phyton.2018.87.105