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ARTICLE
Effect of the scarification methods on the germination and growth of seeds of the Fabaceae family members in presence of silver nanoparticles
Vázquez Núñez E1, S Awate2, MC Carrasco Monrroy3
1 Department of Chemical, Electronics and Biomedical Engineering, University of Guanajuato. Lomas del Bosque 103, León, Guanajuato, México, MX37150
2 Centre for Environment Education, Central Regional Cell, 167/1 New DP Road, Aundh, Pune 411007, India
3 Environmental Engineering Department, Universidad Tecnológica de Tula-Tepeji, Tula de Allende, Hidalgo, México, MX43830.
Address correspondence to: Edgar Vázquez Núñez, tel: +52 473 732 00 06; e-mail:
Phyton-International Journal of Experimental Botany 2018, 87(all), 216-224. https://doi.org/10.32604/phyton.2018.87.216
Abstract
The effect of scarification methods and silver nanoparticles
on plantlets growth were tested on seeds of plants belonging
to the Fabaceae family (
Prosopis laevigata, Acacia farnesiana and
Erythrina americana), which are predominant species in semi-arid
ecosystems in Mexico. The scarification methods consisted in using
coarse sand paper and two different concentrations of sulphuric acid
(H
2SO
4 98% and H
2SO
4 50%); immersion of seeds in distilled water
was used as a control. The percentage of germination was calculated
and the Kotowski´s coefficient was determined. After scarification,
the seeds were immersed in silver nanoparticles solutions at different
concentrations i.e., 100 mg/L, 500 mg/L and 1000 mg/L. Thereafter,
seeds were incubated in Petri dishes. Root and shoot lengths and
dry biomass were measured at 7, 14 and 30 days. The mechanical
scarification showed the maximum level of germination in the three
tested plants. A negative effect was observed on the root/shoot of the
plantlets exposed to silver nanoparticles solutions at different concentrations.
Keywords
Cite This Article
E, V. N., Awate, S., Monrroy, M. C. (2018). Effect of the scarification methods on the germination and growth of seeds of the Fabaceae family members in presence of silver nanoparticles.
Phyton-International Journal of Experimental Botany, 87(all), 216–224.