Open Access
ARTICLE
The Potassium Transporter AtKUP12 Enhances Tolerance to Salt Stress through the Maintenance of the K+/Na+ Ratio in Arabidopsis
Hua Zhang#, Zhongmin Yang#, Xilong You, Youqiang Heng, Yan Wang*
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology,
Xinjiang University, Urumqi, 830046, China
* Corresponding Author: Yan Wang. Email:
# These authors have contributed equally to this work
(This article belongs to this Special Issue: Plant Physiology for Crop Production and Sustainable Agriculture)
Phyton-International Journal of Experimental Botany 2021, 90(2), 389-402. https://doi.org/10.32604/phyton.2021.014156
Received 03 September 2020; Accepted 25 October 2020; Issue published 07 February 2021
Abstract
Potassium (K
+) is a necessary nutrient for plant growth and crop production. The K
+ transporter plays crucial roles in the absorption and transport
of K
+ in plants. Most K
+ transporters in
Arabidopsis have been reported, but AtKUP12, which is a member of the KT/KUP/HAK family, has not yet been the subject of relevant in-depth research. In the present study, we demonstrated that
AtKUP12 plays a crucial role in K
+ uptake in
Arabidopsis under 100 μM lowK
+ and 125 mM salt stress conditions.
AtKUP12 transcripts were induced by
K
+ deficiency and salt stress. We analyzed the K
+ uptake of AtKUP12 using
the K
+ uptake-deficient yeast R5421 and
Arabidopsis mutant
atkup12. Transformation with
AtKUP12 rescued the growth defect of mutant yeast and
atkup12
mutant plants at the low-K
+ concentration, which suggested that AtKUP12 might
be involved in high-affinity K
+ uptake in low-K
+ environments. In comparison to
the wild-type (WT) and
atkup12-AtKUP12 complementation lines,
atkup12
showed a dramatic reduction in potassium concentration, K
+/Na
+ ratio, and root
and shoot growth on 12-day-old seedlings under the salt conditions; however,
there was no significant difference between the complementation and WT lines.
Taken together, these results demonstrate that AtKUP12 might participate in salt
tolerance in
Arabidopsis through K
+ uptake and K
+/Na
+ homeostasis.
Keywords
Cite This Article
Zhang, H., Yang, Z., You, X., Heng, Y., Wang, Y. (2021). The Potassium Transporter AtKUP12 Enhances Tolerance to Salt Stress through the Maintenance of the K
+/Na
+ Ratio in
Arabidopsis.
Phyton-International Journal of Experimental Botany, 90(2), 389–402. https://doi.org/10.32604/phyton.2021.014156