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Diversity Analysis of Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index of Tea Based on Dualex

Yifu Liu1,2, Jiazhen Wang1,2,*, Yao Xiao1, Xiongwen Shi1, Yixia Zeng1

1 College of Biology and Agriculture Science and Technology, Zunyi Normal University, Zunyi, 563000, China
2 Tea Research Institute, Zunyi Normal University, Zunyi, 563000, China

* Corresponding Author: Jiazhen Wang. Email:

(This article belongs to this Special Issue: Plant Bioactive Compounds-Chemotaxonomic Significance, Antioxidant Properties, Potential Application as Food Ingredients as well as Therapeutics, and the Analytical Technology in Their Efficient Discovery)

Phyton-International Journal of Experimental Botany 2021, 90(5), 1549-1558.


The chemical compositions of tea (Camellia sinensis) are affected by numerous factors, such as cultivar, climate, leaf position, and cultivation pattern. However, under the same conditions, the chemical compositions are mainly determined by varieties. Therefore, we investigated the genetic diversity of chemical compositions of tea tree resources in China to screen excellent germplasm resources. Three chemical compositions index (including chlorophyll index, flavonoid index, and anthocyanin index) and the nitrogen balance index of tea leaves were measured in 102 tea germplasms planted in Chinese Tea Plants Improved Variety Germplasm Resources Nursery (CTPIVGRN) by Dualex on April 15 2019. Results showed that the chlorophyll, flavonoid, and anthocyanin contents and the nitrogen balance index significantly differed between the 102 tea germplasms. The genetic diversity index values were 2.005, 2.246, 1.599, and 1.838, and the average genetic diversity was 1.922. The 102 tea germplasms can be divided into four categories by cluster analysis under the genetic distance threshold of 11. These results suggest that the genetic diversity of tea germplasm resources in China is rich. This study’s results can serve as a basis for the diversified development and utilization of tea plant.


Cite This Article

Liu, Y., Wang, J., Xiao, Y., Shi, X., Zeng, Y. (2021). Diversity Analysis of Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index of Tea Based on Dualex. Phyton-International Journal of Experimental Botany, 90(5), 1549–1558.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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