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Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents

Shugufta Parveen1, Fayaz A. Mohiddin2,*, M. Ashraf Bhat3, Zahoor Ahmed Baba4, Fehim Jeelani5, M. Anwar Bhat6, Sajad Un Nabi7, Burhan Hamid2, Saba Bandey8, Farhanaz Rasool9, Zakir Amin1, Ibrahim Al-Ashkar10,*, Muhammad Adnan11, Ayman El Sabagh12

1 Division of Plant Pathology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, India
2 Mountain Research Centre for Field Crops, SKUAST-Kashmir, Khudwani, India
3 Division of Genetics and Plant Breeding, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, India
4 Division of Microbiology, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, India
5 Division of Statistics, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, India
6 Division of Agronomy, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, India
7 Indian Council of Agricultural Research-Central institute of Temperate Horticulture (ICAR-CITH), Old Airport, Rangreth, Srinagar, India
8 Division of Plant Pathology, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, India
9 Directorate of Extension, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, India
10 Department of Plant Production, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia
11 College of Food, Agricultural and Environmental Sciences, The Ohio State University, Columbus, OH, USA
12 Department of Agronomy, Faculty of Agriculture, University of Kafrelsheikh, Kafrelsheikh, Egypt

* Corresponding Authors: Fayaz A. Mohiddin. Email: email; Ibrahim Al-Ashkar. Email: email

Phyton-International Journal of Experimental Botany 2023, 92(11), 3021-3041. https://doi.org/10.32604/phyton.2023.030921

Abstract

One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL−1) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM mL−1). In terms of growth promotion traits, Aspergillus flavus and Enterobacter cloacae excelled in activities viz, phosphorous solubilization, ammonia production, auxin and gibberellic acid production, and nitrogen fixation. The Identity of these endophytes was confirmed through molecular analysis as Trichoderma afroharzianum, Trichoderma harzianum, Penicillium rubens, Aspergillus flavus, Stenotrophomonas rhizophila, Stenotrophomonas maltophilia, Bacillus cereus, Enterobacter cloacae, and Bacillus licheniformis. Under greenhouse conditions, the highest disease control was shown by isolate Bacillus licheniformis and A. flavus with an inhibition of 79%, followed by S. rhizophila (77%) and T. afroharzianum (73%). The overall results of this study showed that Bacillus licheniformis and Stenotrophomonas rhizophila have great potential to be used as bio-stimulant and biocontrol agents to manage rice blast disease.

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APA Style
Parveen, S., Mohiddin, F.A., Bhat, M.A., Baba, Z.A., Jeelani, F. et al. (2023). Characterization of endophytic microorganisms of rice (oryza sativa L.) potentials for blast disease biocontrol and plant growth promoting agents. Phyton-International Journal of Experimental Botany, 92(11), 3021-3041. https://doi.org/10.32604/phyton.2023.030921
Vancouver Style
Parveen S, Mohiddin FA, Bhat MA, Baba ZA, Jeelani F, Bhat MA, et al. Characterization of endophytic microorganisms of rice (oryza sativa L.) potentials for blast disease biocontrol and plant growth promoting agents. Phyton-Int J Exp Bot. 2023;92(11):3021-3041 https://doi.org/10.32604/phyton.2023.030921
IEEE Style
S. Parveen et al., "Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents," Phyton-Int. J. Exp. Bot., vol. 92, no. 11, pp. 3021-3041. 2023. https://doi.org/10.32604/phyton.2023.030921



cc Copyright © 2023 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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