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Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.

Yanhua Li1,#, Hualei Huang1,#, Youming Shi1, Shuqin Huang1, Tao Liu1, Changming Xiao1, Xiaoqing Tian2, Ping Zhao1, Xiaoyan Dai3, Taocui Huang1,*, Yan Zhou1,*
1 Chongqing Academy of Agricultural Sciences, Chongqing, 401329, China
2 Chongqing Municipal Agricultural School, Chongqing, 401329, China
3 Fengdu Agricultural Technology Service Center, Chongqing, 408201, China
* Corresponding Authors: Taocui Huang. Email: ; Yan Zhou. Email:
(This article belongs to this Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)

Phyton-International Journal of Experimental Botany 2023, 92(3), 815-835. https://doi.org/10.32604/phyton.2023.023928

Received 18 May 2022; Accepted 09 September 2022; Issue published 29 November 2022

Abstract

Gibberellin 2-oxidases (GA2ox) are important enzymes that maintain the balance of bioactive GAs in plants. GA2ox genes have been identified and characterized in many plants, but these genes were not investigated in Brassica napus. Here, we identified 31 GA2ox genes in B. napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes. Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm, and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons. Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups, including two C19-GA2ox and two C20-GA2ox clades. Group 4 is a C20-GA2ox Class discovered recently. Most BnaGA2ox genes had a syntenic relationship with AtGA2ox genes. BnaGA2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A subgenome. BnaGA2ox genes were highly expressed in specific tissues such as those involved in growth and development, and most of them were mainly involved in abiotic responses, regulation of phytohormones and growth and development. Our study provided a valuable evolutionary analysis of GA2ox genes in monocotyledons and dicotyledons, as well as an insight into the biological functions of GA2ox family genes in B. napus.

Keywords

Brassica napus; GA2ox gene family; evolution; expression patterns

Cite This Article

Li, Y., Huang, H., Shi, Y., Huang, S., Liu, T. et al. (2023). Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.. Phyton-International Journal of Experimental Botany, 92(3), 815–835.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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