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Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of Brassica napus

Liang Chai1,2, Cheng Cui1, Benchuan Zheng1, Jinfang Zhang1, Jun Jiang1, Haojie Li1,2,*, Liangcai Jiang1,*

1 Crop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China
2 Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu, 610066, China

* Corresponding Authors: Haojie Li. Email: email; Liangcai Jiang. Email: email

(This article belongs to the Special Issue: Plant Omics in Challenging Environment)

Phyton-International Journal of Experimental Botany 2023, 92(3), 645-663. https://doi.org/10.32604/phyton.2023.024717

Abstract

Nitrogen (N) is one of the basic nutrients and signals for plant development and deficiency of it would always limit the productions of crops in the field. Quantitative research on expression of N-stress responsive proteins on a proteome level remains elusive. In order to gain a deep insight into the proteins responding to nitrogen stress in rapeseed (Brassica napus L.), comparative proteomic analysis was performed to investigate changes of protein expression profiles from the root, stem and leaf under different N concentrations, respectively. More than 200 differential abundance proteins (DAPs) were detected and categorized into groups according to annotations, including “binding and catalytic activity”, “involved in primary metabolism and cellular processes”, “stress-response” and so on. Variation in chlorophyll (Chl) content and antioxidant activities further revealed that oxidative stress raised with the increase of N concentration. Bioinformatics analysis based on the expression level of total proteins suggested these DAPs might play important roles in adaptation to N-stress conditions. Generally, these results provides a new aspect into N-stress responding proteins in Brassica plants.

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APA Style
Chai, L., Cui, C., Zheng, B., Zhang, J., Jiang, J. et al. (2023). Global and comparative proteome analysis of nitrogen-stress responsive proteins in the root, stem and leaf of <i>brassica napus</i>. Phyton-International Journal of Experimental Botany, 92(3), 645-663. https://doi.org/10.32604/phyton.2023.024717
Vancouver Style
Chai L, Cui C, Zheng B, Zhang J, Jiang J, Li H, et al. Global and comparative proteome analysis of nitrogen-stress responsive proteins in the root, stem and leaf of <i>brassica napus</i>. Phyton-Int J Exp Bot. 2023;92(3):645-663 https://doi.org/10.32604/phyton.2023.024717
IEEE Style
L. Chai et al., "Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of <i>Brassica napus</i>," Phyton-Int. J. Exp. Bot., vol. 92, no. 3, pp. 645-663. 2023. https://doi.org/10.32604/phyton.2023.024717



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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