Open Access

ARTICLE

Genome Wide Characterization of CBL-CIPK Family Genes and Their Responsive Expression in Rosa chinensis

Lunzeng Huang^1,2,#, Hongsheng Gao^1,#, Ning Jiang^1,2, Yunhong Xu^1,2, Zijian Gong^1,2, Lele Chen^1,2, Shijie Xue^1,2, Xiaoyan Li¹, Ruichao Liu^1,2, Bei Li¹, Hongxia Zhang¹, Chunyan Yu^1,*, Xiaotong Guo^1,2,*

1 The Engineering Research Institute of Agriculture and Forestry, Ludong University, Yantai, 264025, China
2 College of Agriculture, Ludong University, Yantai, 264025, China

* Corresponding Authors: Chunyan Yu. Email: ; Xiaotong Guo. Email:

Phyton-International Journal of Experimental Botany 2023, 92(2), 349-368. https://doi.org/10.32604/phyton.2022.025467

Received 14 July 2022; Accepted 29 August 2022; Issue published 12 October 2022

Abstract

Calcium (Ca²⁺) plays a pivotal role in various signal transduction pathways. Calcineurin B-like proteins (CBLs) are a unique group of Ca²⁺ sensors that decode Ca²⁺ signals by activating the plant specific protein kinase known as the CBL-interacting protein kinase (CIPK). In plants, the CBL-CIPK signaling network regulates multiple signals in response to different extracellular cues including abiotic stress. However, the genome wide annotation and expression patterns of CBLs and CIPKs in woody cutting flower plants are still unclear. In this study, a total number of 7 CBLs (RcCBLs) and 17 CIPKs (RcCIPKs) genes, divided into four and five subfamilies, respectively, were identified from the rose genome. All RcCBLs possess a classic elongation factor-hand (EF-hand) domain, while all RcCIPKs possess both the classic kinase and NAF domains. Most RcCBLs were predicted to be plasma membrane localized, whereas most RcCIPKs were predicted to be cytoplasmic localized. Synteny analysis showed that one RcCBL gene pair and five RcCIPK gene pairs have gone through whole genome duplication events. Promoter cis-element prediction assays indicated that RcCBLs and RcCIPKs could function in different abiotic stress responses in rose plants. Further quantitative real-time PCR analysis demonstrated that RcCBLs and RcCIPKs were expressed in different organs with overlapped but distinct patterns in response to various abiotic stresses. The findings in this work will provide fundamental information and gene resources for further functional research on RcCBLs and RcCIPKs.

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Keywords

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Supplementary Material

Supplementary Material File

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