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Effects of Manganese on the Antioxidant System and Related Gene Expression Levels in the “Hong Yang” Kiwifruit Seedlings

Chongpei Zheng1, Liangliang Li2, Zhencheng Han2, Weijie Li2,*, Xiaopeng Wen1,*

1 Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang, 550025, China
2 Institute of Mountain Resource, Guizhou Academy of Sciences, Guiyang, 550001, China

* Corresponding Authors: Weijie Li. Email: email; Xiaopeng Wen. Email: email

(This article belongs to the Special Issue: New Approaches to Mitigate Abiotic and Biotic Stresses for Improving Crop Productivity and Quality)

Phyton-International Journal of Experimental Botany 2023, 92(8), 2399-2412. https://doi.org/10.32604/phyton.2023.029891

Abstract

To explore how manganese affects the antioxidant system and the expression levels of related genes of “Hong yang” seedlings, the leaves of its tissue cultured seedlings were taken as test materials, and single factor treatment was performed by changing the manganese chloride (MnCl2.4H2O) solution concentration when spraying the leaves. The expression levels of Mn-SOD, POD64 and POD27 genes in leaves were quantitatively analyzed by real-time quantitative PCR (qRT-PCR) at different determination times. Meanwhile, the contents of malondialdehyde (MDA), hydrogen peroxide (H2O2), the activities of antioxidant enzymes, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The results showed that the SOD, CAT, POD, ascorbate peroxidase (APX), and reduced glutathione (GSH) activities in leaves were the highest at 12 h post-treatment with 50 μM MnCl2·4H2O. Furthermore, the contents of MDA and H2O2 in leaves also peaked when the concentration of H2O2 is 50 μM, which is the minimum value. Additionally at 50 μM Mn2+, the Mn-SOD and POD27 expression was up-regulated as compared to the control, which promoted the expression of their respective enzyme activities. However, POD64 expression increased with the increasing Mn2+ concentration. Therefore, 50 μM is the optimal concentration of Mn when exogenously applied on “Hong yang”, which improve the antioxidant enzyme activity and regulate the plant’s physiological and biochemical functions.


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APA Style
Zheng, C., Li, L., Han, Z., Li, W., Wen, X. (2023). Effects of manganese on the antioxidant system and related gene expression levels in the “hong yang” kiwifruit seedlings. Phyton-International Journal of Experimental Botany, 92(8), 2399-2412. https://doi.org/10.32604/phyton.2023.029891
Vancouver Style
Zheng C, Li L, Han Z, Li W, Wen X. Effects of manganese on the antioxidant system and related gene expression levels in the “hong yang” kiwifruit seedlings. Phyton-Int J Exp Bot. 2023;92(8):2399-2412 https://doi.org/10.32604/phyton.2023.029891
IEEE Style
C. Zheng, L. Li, Z. Han, W. Li, and X. Wen "Effects of Manganese on the Antioxidant System and Related Gene Expression Levels in the “Hong Yang” Kiwifruit Seedlings," Phyton-Int. J. Exp. Bot., vol. 92, no. 8, pp. 2399-2412. 2023. https://doi.org/10.32604/phyton.2023.029891



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