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In Vitro Synthetic Seed Production of Potato under Different Fungicide Levels and Storage Intervals

Tahira Kalsoom1, Touqeer Ahmed1, Muhammad Azam Khan1, Mirza Hasanuzzaman2,*, Mukhtar Ahmed3,*, Stefaan P. O. Werbrouck4

1 Department of Horticulture, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan
2 Department of Agronomy, Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh
3 Department of Agronomy, PMAS-Arid Agriculture University, Rawalpindi, 46300, Pakistan
4 Department of Plants and Crops, Ghent University, Ghent, 9000, Belgium

* Corresponding Authors: Mirza Hasanuzzaman. Email: email; Mukhtar Ahmed. Email: email

Phyton-International Journal of Experimental Botany 2023, 92(8), 2429-2450. https://doi.org/10.32604/phyton.2023.030846

Abstract

Potato propagation through tubers and seed is an inefficient way of propagation thus we propose an alternative method in this study which is in vitro propagation. Plantlets were raised from True Potato Seeds (TPS) in test tubes containing 10 mL of Murashige Skoog (MS) medium. Subculturing was done after every 21 days and after four subsequent subculturing, when there was an adequate quantity of stock material, uninodal cuttings of about 3–4 mm in length were prepared from in vitro proliferated potato plants by excising leaves of 1–2 mm on each side of the node. Prepared uninodal cuttings were cultured on ½ strength MS medium for root primordia development. The selected uninodal cuttings with root primordia were encapsulated with sodium alginate solution (3.5% (w/v), mixed with 1.25% charcoal and different concentration of fungicide (thiophanate methyl, sigma) to enhance the viability percentage of synthetic seed. Furthermore, these encapsulated uninodal cuttings were subjected to different storage intervals to check maximum storage. Results showed that optimum dose for fungicide is 150 mg/L as it resulted in good viability percentage, shoot number, shoot length, rooting percentage, root number, root length and ideal for bead formation with sodium alginate. Similarly, results revealed that encapsulated uninodal cuttings can be stored at a temperature of 4°C up to 45 days with a survival rate of 63.33%. Encapsulated uninodal cutting stored for 60 days showed a poor viability percentage of about 43.03%. It can be concluded that optimum dose of 150 mg/L with storage time of 45 days should be used to get a better outcome for synthetic potato seed production.

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APA Style
Kalsoom, T., Ahmed, T., Khan, M.A., Hasanuzzaman, M., Ahmed, M. et al. (2023). in vitro synthetic seed production of potato under different fungicide levels and storage intervals. Phyton-International Journal of Experimental Botany, 92(8), 2429-2450. https://doi.org/10.32604/phyton.2023.030846
Vancouver Style
Kalsoom T, Ahmed T, Khan MA, Hasanuzzaman M, Ahmed M, Werbrouck SPO. in vitro synthetic seed production of potato under different fungicide levels and storage intervals. Phyton-Int J Exp Bot. 2023;92(8):2429-2450 https://doi.org/10.32604/phyton.2023.030846
IEEE Style
T. Kalsoom, T. Ahmed, M.A. Khan, M. Hasanuzzaman, M. Ahmed, and S.P.O. Werbrouck "In Vitro Synthetic Seed Production of Potato under Different Fungicide Levels and Storage Intervals," Phyton-Int. J. Exp. Bot., vol. 92, no. 8, pp. 2429-2450. 2023. https://doi.org/10.32604/phyton.2023.030846



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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