Open Access
ARTICLE
Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed (Brassica napus L.) Germplasm with High/Low Linolenic Acid Trait
Haoxue Wu#, Xiaohan Zhang§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu*
College of Agronomy, Northwest A&F University, Yangling, 712100, China
* Corresponding Author: Chengyu Yu. Email:
# The two authors contributed equally
§ Present address: Jiangsu Cowin Biotech Co., Ltd., Zhenjiang, 212000, China
Phyton-International Journal of Experimental Botany 2024, 93(3), 627-640. https://doi.org/10.32604/phyton.2024.050321
Received 02 February 2024; Accepted 26 February 2024; Issue published 28 March 2024
Abstract
Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in
Brassica napus breeding.
B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases
FAD2 and
FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression levels of most
FAD2 and
FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW. The three high-ALA accessions shared similar sequences with the promoters and CDSs of
BnFAD3.C4 and
BnFAD3.A3. In A28 and SW, substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5, an absence of
BnFAD2.C1 locus, and a 549 bp long deletion on the
BnFAD3.A3 promoter were detected. The profile of
BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies. The mutations in
BnFAD3 in the high-ALA accessions are reported for the first time. In identifying the sites of these mutations, we provide detailed information to aid the design of molecular markers for accelerated breeding schemes.
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Cite This Article
APA Style
Wu, H., Zhang, X., Chen, X., Li, K., Xu, A. et al. (2024). Landscape of sequence variations in homologous copies of <i>fad2</i> and <i>fad3</i> in rapeseed (<i>brassica napus</i> L.) germplasm with high/low linolenic acid trait. Phyton-International Journal of Experimental Botany, 93(3), 627-640. https://doi.org/10.32604/phyton.2024.050321
Vancouver Style
Wu H, Zhang X, Chen X, Li K, Xu A, Huang Z, et al. Landscape of sequence variations in homologous copies of <i>fad2</i> and <i>fad3</i> in rapeseed (<i>brassica napus</i> L.) germplasm with high/low linolenic acid trait. Phyton-Int J Exp Bot. 2024;93(3):627-640 https://doi.org/10.32604/phyton.2024.050321
IEEE Style
H. Wu et al., "Landscape of Sequence Variations in Homologous Copies of <i>FAD2</i> and <i>FAD3</i> in Rapeseed (<i>Brassica napus</i> L.) Germplasm with High/Low Linolenic Acid Trait," Phyton-Int. J. Exp. Bot., vol. 93, no. 3, pp. 627-640. 2024. https://doi.org/10.32604/phyton.2024.050321