Chen Chen^#,*, Chunli Li^#, Tengfei Li, Qianhong Li, Luyao Li, Fengqin Liu

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063549

Abstract Lycium ruthenicum Murray, a plant widely cultivated in northwestern China, is integral to traditional Chinese medicine, with applications in treating menstrual disorders, cardiovascular diseases, and menopausal symptoms. Despite its recognized medicinal value and use as a functional food, comprehensive knowledge of its metabolites and their pharmacological effects remains limited. This study presents an innovative approach using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC–QTOF/MS) to conduct a detailed analysis of both wild and cultivated L. ruthenicum samples. A total of 62 peaks were detected in the total ion current profile, with 59 metabolites identified based… More >

Chen Lin^1,*, Zidan Zhang¹, Zhao Zhang¹, Yuxiang Long¹, Xuwen Shen¹, Jinghao Zhang¹, Youping Wang^1,2,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063086

Abstract Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrates in response to various stress conditions. Beyond their role in stress adaptation, certain GSTs are integral regulators of plant growth and development, contributing to a range of physiological processes. Most GST proteins exhibit dual enzymatic activities, functioning as both transferases and peroxidases, which enables their involvement in diverse cellular processes, including detoxification and stress responses. Recent advancements, particularly in X-ray crystallography, have enabled detailed structural analysis of GST More >

Alexis Salazar-Navarro¹, Víctor Ruíz-Valdiviezo², José Joya-Dávila¹, Daniel González-Mendoza^1,*, Olivia Tzintzun-Camacho¹, Ulin Basilio-Cortes¹, Dagoberto Duran-Hernández¹, Henry López-Lopez¹, Onecimo Grimaldo-Juárez¹

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062871

Abstract The biochemical response of Coffea arabica var. Borbon to chitosan and chitosan oligosaccharides (COS) was evaluated in one-year-old plants under greenhouse conditions. COS solutions were synthesized through chemical and physical hydrolysis using acetic acid, hydrogen peroxide, and microwave irradiation. The obtained COS had an average molecular weight (Mw) of 3549.90 ± 0.33 Daltons (Da), a deacetylation degree (DD) of 76.64 ± 1.12%, and a polymerization degree (PD) of 18.91 ± 0.0018. Solutions of chitosan and COS were applied to C. arabica var. Borbon at concentrations of 0.25, 0.5, and 1 wt%. The experimental design was conducted using… More >

Chang Liu^1,3, Chengcheng Yin¹, Jinjin Zhang², Haijun Sun^1,3,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.061790

Abstract To promote the growth of cutting seeding of poplar (Populus L.), nitrogen (N) fertilizer and surface weed managements were required. We here conducted a pot experiment to examine the effects of natural vegetation, barnyardgrass (Echinochloa Beauv.), and sesbania (Sesbania cannabina pers.) on the growth of poplar cutting seedlings, soil properties, and ammonia (NH₃) volatilization under three N inputs (0, 0.5, and 1.5 g/pot, i.e., N0, N0.5, and N1, respectively). Results showed that N application promoted the growth of poplar cutting seedlings, including plant height, ground diameter, and biomass, compared with N0 treatment. Moreover, under N0, sesbania significantly increased… More >

Weiliang Qi^1,2,3,4,*, Wancang Sun⁵, Li Ma⁵, Xiaolong Li¹, Haiqing Liu¹, Cairong Yang⁶, Ziyao Wei¹

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.060712

Abstract Brassica napus L. (B. napus), recognized as a significant cash and oil crop, faces challenges in popularization and application in northern China due to its limited cold resistance. Clarifying the mechanism of cold stress on gene regulation and signal transduction in B. napus is crucial. To address these issues, we conducted transcriptome sequencing and gene expression analysis, along with gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway profiling under natural (25°C) and cold (4°C) conditions in cold tolerant 16VHNTS309 and weak cold-resistant Tianyou 2238 B. napus seedlings. Enhanced genomic annotation was achieved through additional sequencing.… More >

Guolan Wang, Ting Xie, Lijun Fu, Siying Qu, Jie Li^*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.060634

Abstract The objective of this study was to determine the optimal proportions of plant growth regulators for growth and non-tube rapid propagation of Cymbidium tortisepalum var. longibracteatum; seedlings were utilized as the material. The effects of various combinations and concentrations of 6-benzylaminopurine (6-BA), gibberellic acid (GA₃), and naphthaleneacetic acid (NAA) on growth and non-tube rapid propagation were assessed through a single-factor testing and response surface methodology. The results indicated that 6-BA at 60 mg/L, GA₃ at 150 mg/L, and NAA at 30 mg/L were the most effective concentrations for promoting leaf buds formation in the single-factor analysis. Response surface… More >

Hamada Imtara^1,2,*, Feras Abujaber¹, Faady Siouri¹, Aziz Tumeh¹, Bashar Saad²

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063403

Abstract Traditional Palestinian medicine uses Thymus capitatus (T. capitatus), a plant recognized for its therapeutic properties due to its high concentration of essential oils such as thymol and carvacrol, to treat skin diseases, gastrointestinal disorders, and respiratory infections. The present study was conducted to evaluate the antioxidant and anticancer activities of T. capitatus essential oil (EO). Moreover, this study employed computational methods including ADMET analysis and molecular docking. Using Gas chromatography-mass spectrometry (GC-MS) analysis, the phytochemical composition of T. capitatus essential oil was identified. The DPPH scavenging method was used to assess antioxidant activity. The Michigan Cancer Foundation-7 (MCF-7) and… More >

Ahmed Qasem^*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062683

Abstract Pimpinella anisum, commonly known as anise, is generally used in both folk medicine and the culinary world. In traditional medicine, it is valued for its digestive, respiratory, and antispasmodic properties. This study aims to examine the volatile compounds and antibacterial effect of P. anisum essential oil (PAEO) as well as for the first time its genotoxicity employing both in vitro and computational approaches. Gas chromatography-mass spectrometry (GC-MS) analysis identified anethole as the principal compound, which comprises 92.47% of PAEO. PAEO was tested for its potential antibacterial properties against Bacillus subtilis ATCC 6633, Listeria innocua ATCC 33090, Staphylococcus aureus ATCC 29213, Klebsiella aerogenes… More >

Rui Zhang¹, Le Ren¹, Zhihang Liu¹, Xinyue Gu¹, Shanshan Du¹, Xiao Chen¹, Bingran Xi¹, Bingnan Zhao^1,2, Chao Si^1,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062281

Abstract Plant invasion poses a serious environmental challenge to freshwater ecosystems. Heavy metal pollution and nutrient enrichment, particularly nitrogen (N) and phosphorus (P), are two additional critical environmental issues known to influence the growth of invasive plants. Notably, the N/P ratio in the environments is often inconstant; however, whether these variations can cause differential responses in invasive plants remains underexplored, despite its crucial importance for understanding plant invasiveness. In this study, we examined the effects of different N/P ratios (4:1, 16:1, 32:1, 64:1) on the growth of a widespread invasive plant Alternanthera philoxeroides in cadmium (Cd) polluted… More >

Antonio Rodrigues da Cunha Neto¹, Alexandra dos Santos Ambrósio¹, Arlinda de Jesus Rodrigues Resende¹, Breno Régis Santos¹, Michele Carla Nadal^2,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.061776

Abstract Cytokinins are plant hormones that are essential for plant growth and development and are involved in a variety of processes. They are synthesized by the modification of adenine with an isoprenoid chain, resulting in cytokinins such as isopentenyladenine and zeatin. The levels of these hormones are regulated by conjugation, degradation and oxidation processes that modulate their activity. Cytokinins are perceived by cells through specific receptors that, when activated, trigger signaling cascades responsible for regulating the expression of genes critical for development. In addition, cytokinins interact with other hormones, such as auxins, to coordinate plant growth… More >