Saif Ullah^1,2, Izhar Ali^1,2,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.064608

Abstract Nitrogen (N) assimilation is crucial for the growth and development of C₃ plants, as it converts inorganic N into organic forms, important for protein synthesis, nucleic acids and other vital biomolecules. However, abiotic stressors such as drought, salinity, extreme temperatures and others significantly impact N uptake and utilization, thereby hindering plant growth and development. Recent advances in molecular biology have illuminated the complex networks that govern N assimilation under these stressful conditions, emphasizing the role of transcription factors, regulatory genes, and stress-responsive pathways. This review provides an integrated perspective on the latest research in nitrogen metabolism… More >

Arpita Rani Roy¹, Israt Jahan², Sharah Jabeen Mou², Md. Farhan Hasin³, Prodipto Bishnu Angon^1,*, Rebeka Sultana², Badhon Mazumder⁴, Md. Arif Sakil^5,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063675

Abstract Global warming is making plants more susceptible to heat stress. Hence, adjustments to crop production systems are required for global food security. Heat stress (HS) poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development. The degree to which HS affects physiological disruptions, physical harm, and biochemical changes at various growth stages directly correlates with its effects on physiological functions, plant growth, and crop production. One promising approach is soil modification using biochar, which enhances soil health and promotes the development of microbial communities, ultimately… More >

Dandan Wang^*, Jiaxin Chen, Yanwen Zhang

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063894

Abstract Taxus cuspidata, a rare species of the Taxus genus, and its wild resources are under severe threat. The development of cultivated species has become an important strategy to replace wild species. The objective of this work was to elucidate the differences in secondary metabolite accumulation, particularly in the paclitaxel biosynthesis pathway, between wild and cultivated species. This study employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA sequencing (RNA-Seq) technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T. cuspidata. The results showed that the content of paclitaxel in cultivated species was significantly higher… More >

Kesu Wei¹, Guangju Liu², Bin Wei³, Qifang Zhang⁴, Shengjiang Wu¹, Zhaowei Li^2,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062385

Abstract To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased… More >

Shahin Imran^1,2,#,*, Mousumi Jahan Sumi^3,#, Israt Jahan Harine⁴, Newton Chandra Paul¹, Md. Asif Mahamud⁵, Rakibul Hasan Md. Rabbi⁵, Marian Brestic⁶, Mohammad Saidur Rhaman^7,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.064046

Abstract Heavy metal (HM) accumulation in soil poses a major hazard to both ecological health and plant growth progressions. Cadmium (Cd), lead (Pb), copper (Cu), chromium (Cr), arsenic (As), zinc (Zn), and nickel (Ni) are examples of HMs that negatively impact the growth and development of plants, resulting in lower agricultural output and food safety concerns. Biochar (BC), a substance rich in carbon that is formed by pyrolyzing natural biomass, has demonstrated remarkable promise in reducing HM stress in polluted soils. Research has shown that BC effectively lowers plant uptake of metals, and enhances soil qualities,… More >

Yuhang Li¹, Li Sun¹, Tao Wang¹, Bingjun Yu², Zhihong Gao³, Xiaochun Shu¹, Tengyue Yan¹, Weibing Zhuang^1,2,*, Zhong Wang^1,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063647

Abstract bHLH transcription factors, widely exist in various plants, and are vital for the growth and development of these plants. Among them, many have been implicated in anthocyanin biosynthesis across various plants. In the present study, a PdbHLH57 gene, belonging to the bHLH IIIf group, was characterized, which was isolated and cloned from the colored-leaf poplar ‘Zhongshancaiyun’ (ZSCY). The cDNA sequence of PdbHLH57 was 1887 base pairs, and the protein encoded by PdbHLH57 had 628 amino acids, the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa, respectively. Through bioinformatics analysis, PdbHLH57 has been classified… More >

Mohamed reda Kachmar^1,2, Toufik Bouddine³, Abdelaziz Ed-dra^4,5, Safaâ Kachmar⁶, Hammou Anarghou^7,8, Sulaiman Mohammed Alnasser⁹, Fahad M. Alshabrmi¹⁰, Samiah Hamad Al-Mijalli^11,*, Emad M. Abdallah^12,*, Nidal Naceiri Mrabti¹³, Abdelmounaim Laabar¹⁴, Mourad Chikhaoui¹⁵, Galman Aziz^1,4, Lhoussain Hajji³, Said Chakir²

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062289

Abstract Juniperus oxycedrus (J. oxycedrus) is a traditional culinary spice and medicinal herb with a longstanding history of ethnopharmacological applications across diverse cultures. While prior research has explored the biological activities and phytochemical constituents of extracts derived from its leaves and seed cones, the present study systematically investigates their mineral and phenolic profiles alongside their multifunctional bioactive potential. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) analysis revealed a substantial abundance of essential macro- and microelements. Reversed-phase high-performance liquid chromatography (RP-HPLC) further identified high concentrations of phenolic acids (e.g., p-coumaric acid) and flavonoids (e.g., rutin and quercetin). The extracts exhibited More >

Chengyi Zou¹, Sara Zafar^2,*, Umbreen Bibi², Manzar Abbas³, Zuhair Hasnain^4,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062560

Abstract Soil metal pollution is a global issue due to its toxic nature affecting ecosystems and human health. This has become a concern since metals are non-biodegradable and toxic. Most of the reclamation methods currently used for soils rely on the use of physical and chemical means, which tend to be very expensive and result in secondary environmental damage. However, microbe-aided phytoremediation is gaining attention as it is an eco-friendly, affordable, and technically advanced method to restore the ecosystem. It is essential to understand the complex interaction between plants and microbes. The primary function of plant… More > Graphic Abstract

Xinxin Chen¹, Fuxin Li¹, Jingyu Jia¹, Yajing Tian¹, Xin Li^1,2,3,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.062015

Abstract In plants, transcription factor (TF) family LATERAL ORGAN BOUNDARIES DOMAIN (LBD) has been identified to be involved in the ripening and senescence processes of fruits. However, the function of LBD in Hylocereus undatus (Haw.) Britton & Rose (H. undatus) has not been reported yet. Through transcriptomic analysis, virus-induced gene silencing (VIGS) technology, and RT-qPCR validation, we investigated the role of the LBD TFs in the senescence of H. undatus. Transcriptomic analysis revealed that HuLBD1 is a key transcription factor of the LBD family regulating H. undatus senescence. After silencing HuLBD1, 5075 differentially expressed genes (DEGs) were identified. GSEA results showed… More >

Maryam¹, Mohamed M. El-Mogy², Muhammad Faheem Jan³, Iram Naz¹, Ishtiaq Ahmad^4,*, Riaz Ahmad⁵, Muhammad Tanveer Altaf⁶

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2025.063763

Abstract Various environmental stressors, such as salinity, heat, drought, and metals, present significant obstacles to crop productivity. This study delves into the adverse effects of metals, specifically focusing on cadmium (Cd), nickel (Ni), mercury (Hg), chromium (Cr), arsenic (As), lead (Pb), and copper (Cu) on plants. It explores the sources of these metals, examining both natural occurrences and human-induced activities, and investigates the mechanisms through which plants absorb them. Metal pollution, in particular, negatively affects plant and microbiome well-being, producing reactive oxygen species (ROS) that harm essential macromolecules. Traditional stress-resistant plant varieties necessitate substantial development, leading… More >