Enyan Chen^1,#, Jingyu Jia^1,3,#, Jiaju Sun¹, Jie Wang², Xinxin Chen¹, Xin Li^1,3,4,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.057932

Abstract During post-harvest storage of Cucumis sativus fruit, the application of trypsin treatment could increase flavonoid compound levels and reduce oxidative damage. To investigate the mechanism of trypsin-induced flavonoid biosynthesis in C. sativus, we conducted a combined analysis of transcriptomics and widely targeted metabolomics. One hundred and seventy-five significantly different metabolites were obtained from metabolomics data. The kyoto encyclopedia of genes and genomes (KEGG) functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway. By combining the results of the weighted gene co-expression network analysis (WGCNA) with the 130 upregulated phenylpropanoid metabolites, 22… More >

Ziao Wang^1,3,4,#, Likun An^1,3,4,#, Yongmei Cui^1,2,3,4, Yixiong Bai^1,3,4, Guangping Du¹, Kunlun Wu^1,2,3,4,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.055852

Abstract In this study, we analyzed the agronomic and physiological indicators of the leaves and roots of 60 hulless barley varieties under low-temperature treatment, identified the crucial indicators that can reflect the ability of hulless barley to tolerate low-temperature, and evaluated the ability of different hulless barley varieties to tolerate low-temperature. The results indicated significant differences in the agronomic and physiological indicators of 60 hulless barley varieties subjected to low-temperature treatment. Most of the agronomic indicators significantly decreased, whereas most of the physiological indicators significantly increased. However, the magnitude of changes in each agronomic and physiological… More >

Md. Tahjib-Ul-Arif^1,2,*, Md Asaduzzaman³, Bir Jahangir Shirazy^2,4, Md. Shihab Uddine Khan⁵, A. M. Sajedur Rahman^2,6, Yoshiyuki Murata², Sozan Abdel Hamed⁷, Arafat Abdel Hamed Abdel Latef^8,9,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.058710

Abstract Chilling is one of the major abiotic stresses for plants, especially for rice cultivation. Many essential metabolic processes for growth and development are temperature-dependent. In that case, reducing the negative effects of cold stress using exogenous chemicals is a possible option. Therefore, the current study examined the effects of pre-sowing seed treatment with different chemicals, viz. hydrogen peroxide (H₂O₂), salicylic acid (SA), calcium chloride (CaCl₂), thiourea (TU), and citric acid (CA) on the germination of rice seeds (cv. BRRI dhan28) under chilling environments. Rice seeds were soaked in distilled water (control), 10 mM CA, 2 mM SA,… More >

Oleh Koshovyi^1,2,*, Janne Sepp¹, Valdas Jakštas³, Vaidotas Žvikas³, Karina Tolmacheva⁴, Igor Kireyev⁴, Jyrki Heinämäki¹, Ain Raal^1,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056536

Abstract Pineappleweed (Matricaria discoidea DC., Asteraceae) herb is an essential oil containing raw material with spasmolytic and anti-inflammatory activity. It is also rich in phenolics, which may be used in pharmaceutical practice. This study aimed to investigate the phenolic and amino acid composition and the hyporific and analgesic effects of the M. discoidea aqueous-ethanolic extract and its amino acid modifications. In addition, we developed a polyethylene oxide gel formulation with M. discoidea extracts for the 3D-printed oral solid dosage preparations. In M. discoidea extracts, 16 phenolic substances and 14 amino acids were established. The extract and its amino acid preparations More >

Sejin Choi^1,2, Ho-Seok Lee^1,2,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.058012

Abstract Plants interact with a complex network of microorganisms, forming a dynamic holobiont that is crucial for their health, growth, and adaptation. This interconnected system is deeply influenced by environmental factors, which modulate the relationships within the plant microbiome. Key environmental drivers such as light, temperature, and moisture can alter the balance of these interactions, impacting plant immunity, resilience, and overall fitness. The traditional disease triangle model, which emphasizes plant-pathogen-environment interactions, is enhanced by incorporating the role of the microbiome, revealing how microbial communities contribute to disease outcomes. This review highlights the importance of shifting focus More >

Gaoyin Wu^1,#, Yundou Pang^1,#, Ya Tan¹, Zhongcheng Peng¹, Shuanggui Geng¹, Guanglin Tao², Qiuying Li¹, Shuang Wang¹, Lihong Zhang¹, Quanyan Tian¹, Yingliang Liu^1,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056875

Abstract To establish an efficient regeneration method for the rare and endangered plant Mussaenda anomala to address problems regarding its reproductive obstacles and scarce populations. In this study, the terminal buds, axillary buds, stem segments with two axillary buds, stem segments with two axillary buds and one terminal bud, and leaves of M. anomala were used as explants. The effects of different explants and disinfection methods, plant growth regulators and substrates on plant regeneration were explored. The following results were obtained: (1) The terminal bud was a suitable explant for M. anomala tissue culture, and the disinfection method utilized… More >

Xiaodong Shi^*, Tingyu Wang, Sui Ai, Jiasi Li

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056606

Abstract Cotton rose (Hibiscus mutabilis) is a well-known ornamental plant that produces large flowers of vibrant colors. However, metabolites in H. mutabilis flowers with vibrant color have not been fully understood. By performing a combined analysis of metabolomics and transcriptomics data, we here explored mechanisms for the production of primary active compounds in this plant. Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers, with pink flowers exhibiting a greater flavonoid abundance. The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation. On examining the expression of genes related to the structure More > Graphic Abstract

Qianshuo Liu, Jun Zhao^*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056054

Abstract Traditional machine vision algorithms have difficulty handling the interference of light and shadow changes, broken rows, and weeds in the complex growth circumstances of soybean fields, which leads to erroneous navigation route segmentation. There are additional shortcomings in the feature extractFion capabilities of the conventional U-Net network. Our suggestion is to utilize an improved U-Net-based method to tackle these difficulties. First, we use ResNet’s powerful feature extraction capabilities to replace the original U-Net encoder. To enhance the concentration on characteristics unique to soybeans, we integrate a multi-scale high-performance attention mechanism. Furthermore, to do multi-scale feature… More >

Hossam S. El-Beltagi^1,2,*, Mohamed Fathi El-Nady³, Adel A. Rezk^1,4, Abdelaziz M. Tahoon⁵, Mohammed I. Al-Daej¹, Dina Abdulmajid⁶, Mohamed M. El-Mogy⁷, Elsayed Abd Elmaksoud Abomarzoka⁸, Sherif Mohamed El-Ganainy^7,9, Metwaly Mahfouz Salem Metwaly³

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056734

Abstract Rice (Oryza sativa L.) is one of the most important cereal crops in the world. Bakanae disease is a significant rice disease widely distributed in rice-growing regions worldwide. Therefore, the present investigation aimed to assess the optimal concentrations of paclobutrazol (PBZ) as a treatment for rice grains (cv. Sakha 108) to control bakanae disease, also evaluating its impact on grain germination, seedling growth parameters as well as disease index. Paclobutrazol concentrations had no significant impact on seed germination, regardless of whether the seeds were incubated with Fusarium fujikuroi or not. Application of PBZ, either alone or in… More >

Mengyao Wang^1,2,3, Jiao Mao^1,2,3, Yuanmeng Xu^1,2,3, Shu Wang^1,2,3, Yuhan Liu^1,2,3, Jiangling Ren^1,2,3, Sichen Liu^1,2,3, Zhijun Qiao^1,2,3,*, Xiaoning Cao^1,2,3,*

Phyton-International Journal of Experimental Botany, DOI:10.32604/phyton.2024.056157

Abstract To clarify the response characteristics of broomcorn millet yield and quality to various fertilizers under low nitrogen conditions, the present study investigated the effects of different fertilization treatments, including no fertilization (HCK, CCK), urea (HF1, CF1), phosphate fertilizer (HF2, CF2), compound fertilizer (HF3, CF3), and organic fertilizer (HF4, CF4), on the agronomic traits, quality, and starch pasting properties of proso millet. As experimental materials, non-waxy proso millet (Hequ red proso millet, denoted as HQH) and waxy proso millet (Chishu 1, denoted as CS1) were utilized. The results showed that under low nitrogen conditions, urea treatment… More >